Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test.
Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test.
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纤维细胞在早期和长期类风湿关节炎中的作用：一项6个月的试验，包括反复滑膜活检、影像学和肺功能检查。
DOI：
10.1136/rmdopen-2020-001494
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发表时间：
2021-03
期刊：
影响因子：
6.2
通讯作者：
Lindegaard H
中科院分区：
文献类型：
--
作者：
Just SA;Nielsen C;Werlinrud JC;Larsen PV;Hejbøl EK;Tenstad HB;Daa Schrøder H;Barington T;Torfing T;Humby F;Lindegaard H
研究类风湿关节炎（RA）患者外周血、滑膜组织及体外培养中纤维细胞水平与疾病活动性、影像学及肺功能的关系。20例早期RA（ERA）和20例长期RA（LRA）患者参加了为期6个月的前瞻性研究。16例接受腕关节镜检查的患者为健康对照组。RA患者在基线和6个月时对同一手腕进行了肺功能检查、超声和滑液超声引导的针吸活检。在基线（所有）和6个月（ERA）时进行腕关节MRI。通过流式细胞术测量循环纤维细胞，在体外通过分化成纤维细胞的单核细胞的数量测量，并且在滑膜活检中通过在组织切片中计数来测量。纤维细胞主要位于血管周围和滑膜内膜下区域。尽管进行了有效的RA治疗，但纤维细胞水平在试验期间并未下降。在ERA组中，超声评估的滑膜炎增加是中度的，分别与循环和滑膜纤维细胞水平的增加密切相关。试验期间，ERA组通过MRI评估的滑膜炎增加与循环和培养的纤维细胞数量增加中度相关。绝对弥散能力水平总体上与循环和滑膜纤维细胞水平呈弱负相关。试验期间扩散能力的下降与滑膜纤维细胞水平的增加中度相关。我们的研究结果表明，纤维细胞参与RA的发病机制，无论是在滑膜和肺功能的减少，在一部分RA患者。NCT02652299。
To correlate the level of fibrocytes in peripheral blood, synovial tissue and in vitro culture in rheumatoid arthritis (RA) with changes in disease activity, imaging and pulmonary function. Twenty patients with early RA (ERA) and 20 patients with long-standing RA (LRA) were enrolled in a 6-month prospective study. Sixteen patients undergoing wrist arthroscopy were healthy controls. Patients with RA underwent pulmonary function tests, ultrasound and synovial ultrasound-guided needle biopsy of the same wrist at baseline and 6 months. Wrist MRI was performed at baseline (all) and 6 months (ERA). Circulating fibrocytes were measured by flow cytometry, in vitro by the number of monocytes that were differentiated to fibrocytes and in synovial biopsies by counting in histological sections. Fibrocytes were primarily located around vessels and in the subintimal area in the synovium. Fibrocyte levels did not decline during the trial despite effective RA treatment. In the ERA group, increased synovitis assessed by ultrasound was moderate and strongly correlated with an increase in circulating and synovial fibrocyte levels, respectively. Increased synovitis assessed by MRI during the trial in the ERA group was moderately correlated with both increased numbers of circulating and cultured fibrocytes. Absolute diffusion capacity level was overall weakly negatively correlated with the level of circulating and synovial fibrocytes. The decline in diffusion capacity during the trial was moderately correlated with increased levels of synovial fibrocytes. Our findings suggest that fibrocytes are involved in RA pathogenesis, both in the synovium and the reduction in lung function seen in a part of patients with RA. NCT02652299.
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影响因子：
2.3
作者：
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DOI：
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European respiratory review : an official journal of the European Respiratory Society
影响因子：
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