Iron economy in Chlamydomonas reinhardtii.
Iron economy in Chlamydomonas reinhardtii.
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DOI：
10.3389/fpls.2013.00337
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发表时间：
2013-09-02
影响因子：
5.6
通讯作者：
Blaby-Haas CE
中科院分区：
文献类型：
--
作者：
Glaesener AG;Merchant SS;Blaby-Haas CE
虽然植物中铁营养的研究主要集中在铁吸收途径上，但单细胞绿藻莱茵衣藻等光合微生物为了解亚细胞水平的铁代谢提供了极好的实验系统。在这种藻类中已经建立了铁稳态的几个范例，包括叶绿体中的光系统重塑以及响应次优铁供应而优先保留一些途径和关键铁依赖性蛋白质。这篇综述介绍了我们目前对衣藻铁稳态的理解，特别关注对铁供应变化（如缺铁）的特征反应。还概述了研究铁响应基因表达、生理学和代谢的常用方法，包括培养基的制备、细胞大小、细胞密度和菌株选择对定量测量的影响以及测定金属含量和评估铁供应对光合性能影响的方法。
While research on iron nutrition in plants has largely focused on iron-uptake pathways, photosynthetic microbes such as the unicellular green alga Chlamydomonas reinhardtii provide excellent experimental systems for understanding iron metabolism at the subcellular level. Several paradigms in iron homeostasis have been established in this alga, including photosystem remodeling in the chloroplast and preferential retention of some pathways and key iron-dependent proteins in response to suboptimal iron supply. This review presents our current understanding of iron homeostasis in Chlamydomonas, with specific attention on characterized responses to changes in iron supply, like iron-deficiency. An overview of frequently used methods for the investigation of iron-responsive gene expression, physiology and metabolism is also provided, including preparation of media, the effect of cell size, cell density and strain choice on quantitative measurements and methods for the determination of metal content and assessing the effect of iron supply on photosynthetic performance.
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