Conserved developmental transcriptomes in evolutionarily divergent species.
Conserved developmental transcriptomes in evolutionarily divergent species.
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DOI：
10.1186/gb-2010-11-3-r35
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发表时间：
2010
期刊：
影响因子：
12.3
通讯作者：
Shaulsky G
中科院分区：
文献类型：
--
作者：
Parikh A;Miranda ER;Katoh-Kurasawa M;Fuller D;Rot G;Zagar L;Curk T;Sucgang R;Chen R;Zupan B;Loomis WF;Kuspa A;Shaulsky G
盘基网柄菌发育的转录谱揭示了进化上趋异的物种之间转录谱的显着保守性。尽管基因组序列之间存在巨大差异，但进化趋异的生物体通常具有相同的发育解剖结构。社会性变形虫盘基网柄菌和紫盘网柄菌具有相似的发育形态，尽管它们的基因组与人和有颌鱼的基因组一样不同。在这里，我们表明解剖学相似性伴随着广泛的转录组保守性。通过 RNA 测序，我们比较了两个物种中所有转录本的丰度和发育调控。在这两个物种中，大多数基因都受到发育调控，最大的表达变化发生在从单细胞向多细胞的转变过程中。转录的发育调控在两个物种的直向同源物之间高度保守。除了表达时间外，mRNA 产生的水平在直系同源物之间也是保守的，并且与转录物丰度与所需蛋白量相关的直观概念一致。此外，转录组的保守性延伸到细胞类型特异性表达。这些发现表明，考虑到基因组之间巨大的进化距离，发育程序在转录组水平上非常保守。此外，这种转录保守性可能是盘基网柄菌和紫盘基网柄菌相似的发育解剖学的原因。
Transcriptional profiling of Dictyostelium development reveals significant conservation of transcriptional profiles between evolutionarily divergent species. Evolutionarily divergent organisms often share developmental anatomies despite vast differences between their genome sequences. The social amoebae Dictyostelium discoideum and Dictyostelium purpureum have similar developmental morphologies although their genomes are as divergent as those of man and jawed fish. Here we show that the anatomical similarities are accompanied by extensive transcriptome conservation. Using RNA sequencing we compared the abundance and developmental regulation of all the transcripts in the two species. In both species, most genes are developmentally regulated and the greatest expression changes occur during the transition from unicellularity to multicellularity. The developmental regulation of transcription is highly conserved between orthologs in the two species. In addition to timing of expression, the level of mRNA production is also conserved between orthologs and is consistent with the intuitive notion that transcript abundance correlates with the amount of protein required. Furthermore, the conservation of transcriptomes extends to cell-type specific expression. These findings suggest that developmental programs are remarkably conserved at the transcriptome level, considering the great evolutionary distance between the genomes. Moreover, this transcriptional conservation may be responsible for the similar developmental anatomies of Dictyostelium discoideum and Dictyostelium purpureum.
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影响因子：
3
作者：
Raper, KB;Thom, C
通讯作者：
Thom, C
影响因子：
30.8
作者：
Rifkin, SA;Kim, J;White, KP
通讯作者：
White, KP
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作者：
Iranfar, N;Fuller, D;Loomis, WF
通讯作者：
Loomis, WF
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作者：
Maeda, M;Sakamoto, H;Loomis, WF
通讯作者：
Loomis, WF
影响因子：
48
作者：
Mortazavi, Ali;Williams, Brian A.;Wold, Barbara
通讯作者：
Wold, Barbara