Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro.
Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro.
复制标题
DOI：
10.1038/s41598-018-30446-1
复制
发表时间：
2018-08-10
影响因子：
4.6
通讯作者：
Monzen S
中科院分区：
文献类型：
--
作者：
Chiba M;Kubota S;Sato K;Monzen S
胰腺癌在所有癌症中的5年生存率最低。几种细胞外因子参与胰腺癌向远处器官的发展和转移。外泌体是脂质双层、膜封闭的纳米颗粒，被认为是细胞间通讯的重要介质。然而，胰腺癌细胞释放的外泌体在肿瘤微环境中的作用仍然未知。在这里，我们通过体外分析表明，从胰腺癌PK-45 H细胞释放的外泌体激活了人脐静脉内皮细胞（HUVEC）中的各种基因表达。此外，PK-45 H细胞释放的这些外泌体促进Akt和ERK 1/2信号通路分子的磷酸化，并通过HUVEC中动力蛋白依赖性内吞作用促进管形成。我们的研究结果表明，胰腺癌细胞释放的外泌体可能作为一种新的血管生成促进剂。
Pancreatic cancer has the lowest 5 year survival rate among all cancers. Several extracellular factors are involved in the development and metastasis of pancreatic cancer to distant organs. Exosomes are lipid-bilayer, membrane-enclosed nanoparticles that are recognised as important mediators of cell-to-cell communications. However, the role of exosomes released from pancreatic cancer cells in tumour micro-environment remains unknown. Here, we show that exosomes released from pancreatic cancer PK-45H cells activate various gene expressions in human umbilical vein endothelial cells (HUVECs) by in vitro analyses. In addition, these exosomes released from PK-45H cells promote phosphorylation of Akt and ERK1/2 signalling pathway molecules and tube formation via dynamin-dependent endocytosis in HUVECs. Our findings suggested that exosomes released from pancreatic cancer cells may act as a novel angiogenesis promoter.
登录
查看更多内容
影响因子：
4.2
作者：
Chiba M;Kimura M;Asari S
通讯作者：
Asari S
影响因子：
3.7
作者：
Kawamoto T;Ohga N;Akiyama K;Hirata N;Kitahara S;Maishi N;Osawa T;Yamamoto K;Kondoh M;Shindoh M;Hida Y;Hida K
通讯作者：
Hida K
影响因子：
9.8
作者：
Mineo, Marco;Garfield, Susan H.;Taverna, Simona;Flugy, Anna;De Leo, Giacomo;Alessandro, Riccardo;Kohn, Elise C.
通讯作者：
Kohn, Elise C.
影响因子：
11.8
作者：
Macia, Eric;Ehrlich, Marcelo;Kirchhausen, Tomas
通讯作者：
Kirchhausen, Tomas
影响因子：
4.2
作者：
Davis, Rachel;Singh, Karan P.;Shankar, Sharmila
通讯作者：
Shankar, Sharmila