Exosomes secreted from human colorectal cancer cell lines contain mRNAs, microRNAs and natural antisense RNAs, that can transfer into the human hepatoma HepG2 and lung cancer A549 cell lines.
Exosomes secreted from human colorectal cancer cell lines contain mRNAs, microRNAs and natural antisense RNAs, that can transfer into the human hepatoma HepG2 and lung cancer A549 cell lines.
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DOI：
10.3892/or.2012.1967
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发表时间：
2012-11
期刊：
影响因子：
4.2
通讯作者：
Asari S
中科院分区：
文献类型：
--
作者：
Chiba M;Kimura M;Asari S
外切体是从各种细胞释放到细胞外空间的微囊。据报道，外体内的成分因分泌细胞类型的不同而不同。在本研究中，我们研究了Tetraspanin家族蛋白CD63、CD9和CD81作为收集结直肠癌(CRC)细胞系HCT-15、SW480和WiDR外切体的有用标记。此外，我们的目标是检测三种结肠癌细胞系分泌的外切体中的mRNAs、microRNAs和天然反义RNAs。此外，我们检测了含有它们的RNA的外切体是否被转移到肝癌细胞系HepG2和肺癌细胞系A549。3株大肠癌细胞分泌的外切体中均可检测到CD81。这一结果表明，CD81可以作为三个结直肠癌细胞系来源的外切体的收集标记。对3株大肠癌细胞系外切体中的RNA物种进行检测时，分别检测到看家基因ACTB和GAPDH的mRNAs，miR-21、miR-192和miR-221的microRNAs，以及LRRC24、MDM2和CDKN1A基因的天然反义RNAs。在本研究中，我们首次在外体中发现了它们的天然反义RNA。此外，从大肠癌细胞系中提取的PKH67标记的外切体被携带到HepG2和A549细胞中。这些结果表明，包裹在外体中的细胞内RNA被分泌到外部，来自CRC细胞系的外体被转移到HepG2和A549细胞中。综上所述，我们发现从大肠癌细胞系衍生的外切体含有mRNAs、microRNAs和天然的反义RNA，并且可以被运送到HepG2和A549细胞中。这些发现表明，外体RNA可以在细胞之间穿梭，并可能参与受体细胞基因表达的调节。
Exosomes are microvesicles that are released from various cells into the extracellular space. It has been reported that the components within exosomes vary according to the type of secreted cell. In the present study, we investigated the tetraspanin family proteins CD63, CD9 and CD81 as useful collection markers of exosomes derived from the three colorectal cancer (CRC) cell lines HCT-15, SW480 and WiDr. In addition, we aimed to detect the mRNAs, microRNAs and natural antisense RNAs within the exosomes secreted from the three CRC cell lines. Furthermore, we examined whether exosomes containing their RNAs were transferred into the hepatoma cell line HepG2 and lung cancer cell line A549. CD81 was detected in exosomes secreted from the three CRC cell lines. This result indicates that CD81 can be a collection marker of exosomes derived from the three CRC cell lines. When the RNA species within exosomes derived from the three CRC cell lines were examined, the mRNAs of housekeeping genes such as ACTB and GAPDH, the microRNAs such as miR-21, miR-192 and miR-221, and the natural antisense RNAs of LRRC24, MDM2 and CDKN1A genes, were detected. We discovered their natural antisense RNAs within exosomes for the first time in the present study. Furthermore, PKH67-labeled exosomes derived from the CRC cell lines were taken up into HepG2 and A549 cells. These findings indicate that the intracellular RNAs enclosed within exosomes are secreted to the outside, and exosomes derived from the CRC cell lines are transferred into HepG2 and A549 cells. In conclusion, we reveal that exosomes derived from the CRC cell lines contain mRNAs, microRNAs and natural antisense RNAs, and can be delivered into HepG2 and A549 cells. These findings indicate that exosomal RNAs can shuttle between cells, and may be involved in the regulation of gene expression in recipient cells.
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影响因子：
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通讯作者：
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