Changes in NMDA-receptor function in the first week following laser-induced lesions in rat visual cortex.
Changes in NMDA-receptor function in the first week following laser-induced lesions in rat visual cortex.
复制标题
激光诱导大鼠视觉皮层损伤后第一周 NMDA 受体功能的变化
DOI：
10.1093/cercor/bhr318
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Mittmann T
中科院分区：
文献类型：
--
作者：
Imbrosci B;Zhang W;Neubacher U;Hatt H;Eysel UT;Mittmann T
局灶性脑损伤伴随着部分补偿功能丧失的功能重组过程。在先前的研究中，在大鼠视觉皮层中激光诱导的损伤的边缘处的细胞外记录显示出增强的突触可塑性，这是由含有NR 2B的NMDA受体（NMDARs）的活性介导的，从而阐明了这种重组的潜在细胞机制。鉴于NMDARs在功能重组过程中的潜在重要贡献，在本研究中，我们使用相同的病变模型进一步研究病变诱导的功能变化和病变附近NMDARs的定位。最重要的发现是损伤介导的非突触后NMDAR的功能性再表达，但根据我们的数据，突触前或突触内/突触外NMDAR（preNMDAR）在年龄匹配（>P21）的假手术对照中检测不到。值得注意的是，前NMDAR能够促进自发和诱发的突触突触能传递。在突触后部位，我们还发现了NMDAR介导的电流的衰减时间常数的增加，这是伴随着NR 2A/NR 2B的比例降低，如Western印迹分析所揭示的。所有这些研究结果提供了新的见解NMDARs的活动过程中的功能重组局灶性病变后，在大脑皮层的作用。
Focal brain injuries are accompanied by processes of functional reorganization that partially compensate the functional loss. In a previous study, extracellular recordings at the border of a laser-induced lesion in the visual cortex of rats showed an enhanced synaptic plasticity, which was mediated by the activity of NR2B-contaning NMDA-receptors (NMDARs) shedding light on the potential cellular mechanisms underlying this reorganization. Given the potentially important contribution of NMDARs in processes of functional reorganization, in the present study, we used the same lesion model to further investigate lesion-induced changes in function and localization of NMDARs in the vicinity of the lesion. The most important finding was a lesion-mediated functional reexpression of nonpostsynaptic, but according to our data, presynaptic or peri-/extrasynaptic NMDARs (preNMDARs), which were undetectable in age-matched (>P21) sham-operated controls. Notably, preNMDARs were able to boost both spontaneous and evoked synaptic glutamatergic transmission. At the postsynaptic site, we also disclosed an increase in the decay time constant of NMDARs mediated currents, which was accompanied by a decreased NR2A/NR2B ratio, as revealed by Western blot analysis. All together these findings provide new insights into the role of NMDARs activity during processes of functional reorganization following a focal lesion in the cerebral cortex.
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局灶性缺血性病变后视觉皮层图的重组
影响因子：
6.3
作者：
A. Zepeda;L. Vaca;C. Arias;F. Sengpiel
通讯作者：
F. Sengpiel
缺血期间和之后海马选择性脆弱神经元的钙超载：大鼠电子显微镜研究
影响因子：
6.3
作者：
R. Simon;T. Griffiths;M. Evans;J. H. Swan;B. Meldrum
通讯作者：
B. Meldrum
体外视皮层病变周围长时程增强的空间分布
影响因子：
2
作者：
C. Dohle;U. Eysel;T. Mittmann
通讯作者：
T. Mittmann
DOI：
10.1073/pnas.0900068106
发表时间：
2009-05
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Ganna Palagina;U. Eysel;D. Jancke
通讯作者：
Ganna Palagina;U. Eysel;D. Jancke
影响因子：
3.7
作者：
Eysel, UT;Schweigart, G
通讯作者：
Schweigart, G