Beagle pup germinal matrix maturation studies.
Beagle pup germinal matrix maturation studies.
复制标题
小猎犬幼犬生发基质成熟研究。
作者：
Ment,LR;Stewart,WB;Ardito,TA;Madri,JA
脑室内出血，或出血进入发育中的大脑的生殖基质组织，仍然是早产儿的常见问题。这种损伤的“风险期”是出生后的头3-4天。我们假设出血的风险期与围产期生殖基质血管系统的快速成熟有关，并采用新生比格犬模型研究这种成熟。将新生比格犬幼仔（n = 30）麻醉，并用福尔马林缓冲液全身灌注;取出大脑并制备用于免疫组织化学研究。切片染色Bandeiraea凝集素表明，出生后第1天和第4天之间的生殖基质血管密度没有差异。还对骨基质切片的α-平滑肌肌动蛋白、胶原IV、胶原V、结蛋白、因子VIII相关抗原、纤连蛋白、神经胶质细胞酸性蛋白、层粘连蛋白、转铁蛋白和波形蛋白的抗体进行染色。血管染色的α-平滑肌肌动蛋白，直到出生后第10天才注意到，和差异染色检测层粘连蛋白和胶原蛋白V的抗体。定量染色强度的共聚焦显微镜显示，在出生后第4天与第1天相比，细胞外基质成分显着增加（P <0.05）。这些基底膜蛋白可能增加了足够的结构完整性，以防止毛细血管破裂，从而脑室内出血。
Intraventricular hemorrhage, or hemorrhage into the germinal matrix tissues of the developing brain, remains a common problem of preterm infants. The "risk period" for this insult is the first 3-4 postnatal days. We hypothesized that this risk period for hemorrhage is related to rapid perinatal maturation of the germinal matrix vasculature and employed the newborn beagle pup model for the study of this maturation. Newborn beagle pups (n = 30) were anesthetized and systemically perfused with buffered formalin; the brains were removed and prepared for immunohistochemical study. Sections stained with Bandeiraea lectin demonstrated that there was no difference in germinal matrix vessel density between postnatal days 1 and 4. Germinal matrix sections were also stained for antibodies to alpha-smooth muscle actin, collagen IV, collagen V, desmin, factor VIII-related antigen, fibronectin, glial fibrillary acidic protein, laminin, transferrin, and vimentin. Vasculature staining by alpha-smooth muscle actin was not noted until postnatal day 10, and differential staining was detected for antibodies to laminin and collagen V. Quantification of staining intensity by confocal microscopy demonstrated a significant increase in both extracellular matrix components at postnatal day 4 compared with day 1 (p less than 0.05 for both). These basement membrane proteins may add sufficient structural integrity to germinal matrix vessels to prevent capillary rupture and thus intraventricular hemorrhage.
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脑室内出血小猎犬幼犬模型中的局部脑葡萄糖利用。
影响因子：
4.1
作者：
L. Ment;W. Stewart;C. Duncan
通讯作者：
C. Duncan
影响因子：
5.2
作者：
L. D. de Crespigny;R. Mackay;L. Murton;R. Roy;P. H. Robinson
通讯作者：
P. H. Robinson
DOI：
--
发表时间：
1984
期刊：
The Anatomical Record
影响因子：
--
作者：
M. Leuschen;R. Shuman;R. Nelson
通讯作者：
R. Nelson
新生儿的大脑结构和脑室内出血：实时超声研究。
影响因子：
5.2
作者：
M. Levene;J. Wigglesworth;V. Dubowitz
通讯作者：
V. Dubowitz
影响因子：
5.1
作者：
MENT, LR;DUNCAN, CC;GETTNER, P
通讯作者：
GETTNER, P