Fabrication of an electrochemical sensor for determination of doxorubicin in human plasma and its interaction with DNA.
Fabrication of an electrochemical sensor for determination of doxorubicin in human plasma and its interaction with DNA.
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DOI：
10.1016/j.jpha.2016.07.005
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发表时间：
2017-03
影响因子：
8.8
通讯作者：
Shams N
中科院分区：
文献类型：
--
作者：
Hajian R;Tayebi Z;Shams N
在这项工作中，基于多壁碳纳米管修饰铂电极（Pt/MWCNTs）制备了一种电化学传感器，用于检测血浆中蒽环类化疗药物阿霉素（DOX）。在布里顿罗宾逊（B-R）缓冲液（pH 4.0, 0.1 M）中，DOX在修饰电极表面有效积累，并在0.522和0.647 V （vs. Ag/AgCl）附近产生一对氧化还原峰。对pH、缓冲液类型、积累时间、改性剂用量、扫描速率等电化学参数进行了优化。在优化条件下，阴极峰值电流与DOX浓度在0.05 ~ 4.0µg/mL范围内呈线性相关，检出限为0.002µg/mL。电子转移数(n)和电子转移系数（α）分别为2.0和0.25。所构建的传感器对血浆中阿霉素的测定具有良好的精度、灵敏度、重复性和选择性。此外，在DNA存在下，DOX的循环伏安研究表明其嵌入机制的结合常数（Kb）为1.12×105 L/mol。
In this work, an electrochemical sensor was fabricated for determination of an anthracycline, doxorubicin (DOX) as a chemotherapy drug in plasma based on multi-walled carbon nanotubes modified platinum electrode (Pt/MWCNTs). DOX was effectively accumulated on the surface of modified electrode and generated a pair of redox peaks at around 0.522 and 0.647 V (vs. Ag/AgCl) in Britton Robinson (B-R) buffer (pH 4.0, 0.1 M). The electrochemical parameters including pH, type of buffer, accumulation time, amount of modifier and scan rate were optimized. Under the optimized conditions, there was a linear correlation between cathodic peak current and concentration of DOX in the range of 0.05–4.0 µg/mL with the detection limit of 0.002 µg/mL. The number of electron transfers (n) and electron transfer-coefficient (α) were estimated as 2.0 and 0.25, respectively. The constructed sensor displayed excellent precision, sensitivity, repeatability and selectivity in the determination of doxorubicin in plasma. Moreover, cyclic voltammetry studies of DOX in the presence of DNA showed an intercalation mechanism with binding constant (Kb) of 1.12×105 L/mol.
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影响因子：
105.7
作者：
BONADONNA, G;MONFARDINI, S;FOSSATIB.F
通讯作者：
FOSSATIB.F
影响因子：
3
作者：
Evtugyn, Gennady;Porfireva, Anna;Budnikov, Herman
通讯作者：
Budnikov, Herman
影响因子：
8.4
作者：
Goyal, Rajendra N.;Gupta, Vinod K.;Chatterjee, Sanghamitra
通讯作者：
Chatterjee, Sanghamitra
影响因子：
64.8
作者：
IIJIMA, S
通讯作者：
IIJIMA, S
DOI：
10.1016/j.jchromb.2012.01.025
发表时间：
2012-03-01
影响因子：
3
作者：
Alhareth, Khairallah;Vauthier, Christine;Moussa, Fathi
通讯作者：
Moussa, Fathi