Regulation of Crossover Frequency and Distribution during Meiotic Recombination.
Regulation of Crossover Frequency and Distribution during Meiotic Recombination.
复制标题
DOI：
10.1101/sqb.2017.82.034132
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Colaiácovo MP
中科院分区：
文献类型：
--
作者：
Saito TT;Colaiácovo MP
交叉重组是减数分裂过程中产生遗传多样性和促进染色体准确分离的必要条件。交叉重组过程受到严格调控，并由程序性减数分裂DNA双链断裂（DSBs）的形成启动。在大多数物种中，dsb的数量大约是杂交数量的十倍，因为只有有限数量的dsb在减数分裂期间被修复为杂交。此外，交叉并不是随机分布的。大多数交叉位点位于染色体臂区，着丝粒和端粒通常都没有交叉位点。交叉点的丢失或定位错误经常导致染色体不分离和随后的非整倍体，导致不孕、流产和出生缺陷，如唐氏综合症。在这里，我们将回顾在大多数物种中观察到的交叉调控方面，然后重点关注秀丽隐杆线虫的交叉调控，其中交叉的频率和分布都受到严格控制。在这个系统中，每对同源染色体之间只形成一次交叉，通常在偏离中心的位置。我们已经鉴定出具有不受管制交叉分布的秀丽隐杆线虫突变体，我们正在使用可诱导的单DSB系统分析交叉控制，该系统可以在特定基因组位置产生单交叉。这些综合研究揭示了交叉位置如何与准确的染色体分离相关联的新见解。
Crossover recombination is essential for generating genetic diversity and promoting accurate chromosome segregation during meiosis. The process of crossover recombination is tightly regulated and is initiated by the formation of programmed meiotic DNA double-strand breaks (DSBs). The number of DSBs is around ten-fold higher than the number of crossovers in most species, since only a limited number of DSBs is repaired as crossovers during meiosis. Moreover, crossovers are not randomly distributed. Most crossovers are located on chromosomal arm regions and both centromeres and telomeres are usually devoid of crossovers. Either loss or mislocalization of crossovers frequently results in chromosome nondisjunction and subsequent aneuploidy, leading to infertility, miscarriages and birth defects such as Down syndrome. Here, we will review aspects of crossover regulation observed in most species and then focus on crossover regulation in the nematode Caenorhabditis elegans in which both the frequency and distribution of crossovers are tightly controlled. In this system, only a single crossover is formed, usually at an off-centered position, between each pair of homologous chromosomes. We have identified C. elegans mutants with deregulated crossover distribution and we are analyzing crossover control by using an inducible single DSB system with which a single crossover can be produced at specific genomic positions. These combined studies are revealing novel insights into how crossover position is linked to accurate chromosome segregation.
登录
查看更多内容
影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
ATMUS81在A. thaliana中对干扰不敏感的交叉中的作用。
影响因子：
4.5
作者：
Berchowitz, Luke E.;Francis, Kirk E.;Bey, Alexandra L.;Copenhaver, Gregory P.
通讯作者：
Copenhaver, Gregory P.
影响因子：
7.7
作者：
Cortes, Daniel;McNally, Karen;McNally, Francis J.
通讯作者：
McNally, Francis J.
影响因子：
4.5
作者：
Agostinho A;Meier B;Sonneville R;Jagut M;Woglar A;Blow J;Jantsch V;Gartner A
通讯作者：
Gartner A
影响因子：
4.5
作者：
Gao J;Kim HM;Elia AE;Elledge SJ;Colaiácovo MP
通讯作者：
Colaiácovo MP