The role of AtMUS81 in interference-insensitive crossovers in A. thaliana.
The role of AtMUS81 in interference-insensitive crossovers in A. thaliana.
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ATMUS81在A. thaliana中对干扰不敏感的交叉中的作用。
DOI：
10.1371/journal.pgen.0030132
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发表时间：
2007-08
期刊：
影响因子：
4.5
通讯作者：
Copenhaver, Gregory P.
中科院分区：
文献类型：
--
作者：
Berchowitz, Luke E.;Francis, Kirk E.;Bey, Alexandra L.;Copenhaver, Gregory P.
MUS81在植物、动物和真菌中保守，参与有丝分裂DNA损伤修复和减数分裂重组。在这里，我们提出了拟南芥同源基因AtMUS81的功能特征，它在有丝分裂和减数分裂细胞中都有作用。AtMUS81转录本在所有组织中都有产生，但在花药中升高了9倍以上，其水平在伽马辐射和甲烷磺酸甲酯处理后增加。Atmus81转移-DNA插入突变体对广泛的DNA损伤剂表现出更高的敏感性，证实了它在有丝分裂增殖细胞中的作用。为了研究它在减数分裂中的作用，我们采用了一种基于花粉四分体的目测方法。来自1号和3号染色体上遗传区间的数据显示，Atmus81突变体的减数分裂重组略有减少。重要的是，对5号染色体上一对相邻区间内重组的测量表明，Atmus81中剩余的交叉是干扰敏感的，并且Atmus81突变体中的干扰水平显著高于野生型。这些数据与AtMUS81参与干扰不敏感的减数分裂二次子集交换的假设是一致的。减数分裂是一种特殊类型的细胞分裂，其中一个二倍体前体细胞分裂成四个单倍体细胞，这些细胞随后在有性繁殖过程中用于受精。在减数分裂过程中，染色体配对、突触和交换遗传信息，所有这些都是在随后的阶段进行适当的染色体分离所必需的。减数分裂染色体分离不当往往会导致遗传缺陷，如非整倍体。利用模式植物A.thaliana，我们开发了一个功能强大的系统，用于直接在花粉中进行减数分裂重组的可视化分析，在该系统中，单个减数分裂的四个产物融合在一个四分体中。我们已经使用这个系统来表征基因AtMUS81，并表明Atmus81突变体在减数分裂交叉中有适度的减少，并且对广泛的DNA损伤剂敏感。重要的是，Atmus81中剩余的交叉仍然显示出交叉干扰，这是一种现象，即一个交叉会抑制附近其他交叉的发生。我们的结果表明，AtMUS81介导了拟南芥减数分裂重组事件的一个子集，这些重组事件对交叉干扰不敏感。
MUS81 is conserved among plants, animals, and fungi and is known to be involved in mitotic DNA damage repair and meiotic recombination. Here we present a functional characterization of the Arabidopsis thaliana homolog AtMUS81, which has a role in both mitotic and meiotic cells. The AtMUS81 transcript is produced in all tissues, but is elevated greater than 9-fold in the anthers and its levels are increased in response to gamma radiation and methyl methanesulfonate treatment. An Atmus81 transfer-DNA insertion mutant shows increased sensitivity to a wide range of DNA-damaging agents, confirming its role in mitotically proliferating cells. To examine its role in meiosis, we employed a pollen tetrad–based visual assay. Data from genetic intervals on Chromosomes 1 and 3 show that Atmus81 mutants have a moderate decrease in meiotic recombination. Importantly, measurements of recombination in a pair of adjacent intervals on Chromosome 5 demonstrate that the remaining crossovers in Atmus81 are interference sensitive, and that interference levels in the Atmus81 mutant are significantly greater than those in wild type. These data are consistent with the hypothesis that AtMUS81 is involved in a secondary subset of meiotic crossovers that are interference insensitive. Meiosis is a specialized type of cell division in which one diploid progenitor cell divides into four haploid cells that are subsequently used for fertilization during sexual reproduction. During meiosis, chromosomes pair, synapse, and exchange genetic information, all of which are required for proper chromosome segregation during subsequent stages. Failure to properly segregate meiotic chromosomes often leads to genetic defects such as aneuploidy. Using the model plant A. thaliana, we have developed a powerful system for the visual analysis of meiotic recombination directly in the pollen, in which the four products of individual meioses are fused together in a tetrad. We have used this system to characterize the gene AtMUS81 and show that Atmus81 mutants have a moderate reduction in meiotic crossovers and are sensitive to a wide range of DNA-damaging agents. Importantly, the remaining crossovers in Atmus81still exhibit crossover interference, a phenomenon whereby one crossover inhibits the occurrence of other nearby crossovers. Our results suggest that AtMUS81 mediates a subset of meiotic recombination events in Arabidopsis that are insensitive to crossover interference.
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影响因子：
64.5
作者：
Boddy, MN;Gaillard, PHL;Russell, P
通讯作者：
Russell, P
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