Strand invasion of DNA quadruplexes by PNA: comparison of homologous and complementary hybridization.
Strand invasion of DNA quadruplexes by PNA: comparison of homologous and complementary hybridization.
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DOI：
10.1002/cbic.201300263
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发表时间：
2013-08-19
期刊：
影响因子：
--
通讯作者：
Armitage BA
中科院分区：
文献类型：
--
作者：
Gupta A;Lee LL;Roy S;Tanious FA;Wilson WD;Ly DH;Armitage BA
Molecular recognition of DNA quadruplex structures is envisioned as a strategy for regulating gene expression at the transcriptional level and for in situ analysis of telomere structure and function. The recognition of DNA quadruplexes by peptide nucleic acid (PNA) oligomers is presented here, with a focus on comparing complementary, heteroduplex-forming and homologous, heteroquadruplex-forming PNAs. Surface plasmon resonance and optical spectroscopy experiments demonstrated that the efficacy of a recognition mode depended strongly on the target. For a quadruplex derived from the promoter regulatory region found upstream of the MYC proto-oncogene, the homologous PNA readily invades the DNA target to form a heteroquadruplex at high potassium concentration mimicking the intracellular environment, whereas the complementary PNA exhibits virtually no hybridization. In contrast, the complementary PNA is superior to the homologous PNA in hybridizing to a quadruplex modeled on the human telomere sequence. The results are discussed in terms of the different structural morphologies of the quadruplex targets and the implications for in vivo recognition of quadruplexes by PNAs.
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影响因子：
2.9
作者：
Fernando, Himesh;Rodriguez, Raphael;Balasubramanian, Shankar
通讯作者：
Balasubramanian, Shankar
DOI：
10.1038/nrg3296
发表时间：
2012-11
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.9
作者：
Marin, VL;Armitage, BA
通讯作者：
Armitage, BA
影响因子：
14.9
作者：
Ambrus A;Chen D;Dai J;Bialis T;Jones RA;Yang D
通讯作者：
Yang D
影响因子：
14.9
作者：
Fernando H;Sewitz S;Darot J;Tavaré S;Huppert JL;Balasubramanian S
通讯作者：
Balasubramanian S