Synaptobrevin‐2‐like immunoreactivity is associated with vesicles at synapses in rat circumvallate taste buds
Synaptobrevin‐2‐like immunoreactivity is associated with vesicles at synapses in rat circumvallate taste buds
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Synaptobrevin-2 样免疫反应性与大鼠周围味蕾突触囊泡相关
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
J. C. Kinnamon
中科院分区：
文献类型：
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作者：
Ruibiao Yang;Cristi L. Stoick;J. C. Kinnamon
小突触泡蛋白（Synaptobrevin）是一种囊泡相关膜蛋白（VAMP），被认为在中枢神经系统调节突触囊泡对接和神经递质胞吐过程中与突触前膜蛋白（SNAP-25和突触融合蛋白）起关键作用。人们发现味觉细胞和神经突起之间存在突触接触，但对味觉细胞突触处的突触囊泡对接和神经递质释放知之甚少。之前我们证明了对SNAP-25的免疫反应性存在于具有突触的味觉细胞中。我们目前的研究结果表明，synaptobrevin-2-like免疫反应性（-LIR）存在于大鼠轮廓味蕾中大约35%的味觉细胞中。突触小泡蛋白-2-LIR与SNAP-25-、血清素-和蛋白质基因产物9.5-LIR共定位。突触泡蛋白-2-LIR还与III型肌醇1，4，5-三磷酸受体（IP 3R 3）的免疫反应性共定位，IP 3R 3是味觉细胞中的味觉信号分子。所有IP 3R 3-LIR味细胞均表达小突触蛋白-2-LIR。然而，大约27%的小突触泡蛋白-2-LIR味觉细胞不显示IP 3R 3-LIR。我们相信，基于超微结构和生化特征，II型和III型味觉细胞都显示突触泡蛋白-2-LIR。我们观察到的所有从味觉细胞到神经过程的突触都表达小突触蛋白-2-LIR，以及一些没有突触的味觉细胞。通过使用胶体金免疫电镜，我们发现小突触蛋白-2-LIR与大鼠味觉细胞突触的突触囊泡相关。这项研究的结果表明，可溶性NSF附着受体（SNARE）机制可能控制突触囊泡融合和吐出在味觉细胞突触。J. Comp.神经元471：59-71，2004.© 2004 Wiley利斯公司
Synaptobrevin is a vesicle‐associated membrane protein (VAMP) that is believed to play a critical role with presynaptic membrane proteins (SNAP‐25 and syntaxin) during regulated synaptic vesicle docking and exocytosis of neurotransmitter at the central nervous system. Synaptic contacts between taste cells and nerve processes have been found to exist, but little is known about synaptic vesicle docking and neurotransmitter release at taste cell synapses. Previously we demonstrated that immunoreactivity to SNAP‐25 is present in taste cells with synapses. Our present results show that synaptobrevin‐2‐like immunoreactivity (‐LIR) is present in approximately 35% of the taste cells in rat circumvallate taste buds. Synaptobrevin‐2‐LIR colocalizes with SNAP‐25‐, serotonin‐, and protein gene product 9.5‐LIR. Synaptobrevin‐2‐LIR also colocalizes with immunoreactivity for type III inositol 1,4,5‐triphosphate receptor (IP3R3), a taste‐signaling molecule in taste cells. All IP3R3‐LIR taste cells express synaptobrevin‐2‐LIR. However, approximately 27% of the synaptobrevin‐2‐LIR taste cells do not display IP3R3‐LIR. We believe, based on ultrastructural and biochemical features, that both type II and type III taste cells display synaptobrevin‐2‐LIR. All of the synapses that we observed from taste cells onto nerve processes express synaptobrevin‐2‐LIR, as well as some taste cells without synapses. By using colloidal gold immunoelectron microscopy, we found that synaptobrevin‐2‐LIR is associated with synaptic vesicles at rat taste cell synapses. The results of this study suggest that soluble NSF attachment receptor (SNARE) machinery may control synaptic vesicle fusion and exocytosis at taste cell synapses. J. Comp. Neurol. 471:59–71, 2004. © 2004 Wiley‐Liss, Inc.
DOI：
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发表时间：
1995-07
期刊：
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影响因子：
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作者：
A. Spielman;J. Brand
通讯作者：
A. Spielman;J. Brand