Multiple personalities of the RNA polymerase active centre.
Multiple personalities of the RNA polymerase active centre.
复制标题
RNA聚合酶活性中心的多重人格。
DOI：
10.1099/mic.0.079020-0
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zenkin N
中科院分区：
文献类型：
--
作者：
Zenkin N
所有生物体中的转录都是由高度保守的多亚基RNA聚合酶（RNAP）完成的。最近，我们对RNAP活性中心功能的理解发生了变化，发现活性中心附近的保守柔性结构域触发环（TL）直接参与RNA合成的催化，并作为转录保真度的主要决定因素。此外，TL似乎还参与了RNAP交换活性中心催化活性的独特能力。在这种现象中，TL被改变氨基酸含量的转录因子取代，从而改变活性中心的催化性质。存在许多转录因子通过TL的取代起作用，这表明RNAP有几个不同的活性中心可供选择，以响应外部或内部信号。在2014年普通微生物学学会年会上发表的本次颁奖演讲的视频可以通过以下链接观看：https://www.youtube.com/watch? v=79Z7iXVEPo4
Transcription in all living organisms is accomplished by highly conserved multi-subunit RNA polymerases (RNAPs). Our understanding of the functioning of the active centre of RNAPs has transformed recently with the finding that a conserved flexible domain near the active centre, the trigger loop (TL), participates directly in the catalysis of RNA synthesis and serves as a major determinant for fidelity of transcription. It also appears that the TL is involved in the unique ability of RNAPs to exchange catalytic activities of the active centre. In this phenomenon the TL is replaced by a transcription factor which changes the amino acid content and, as a result, the catalytic properties of the active centre. The existence of a number of transcription factors that act through substitution of the TL suggests that the RNAP has several different active centres to choose from in response to external or internal signals.A video of this Prize Lecture, presented at the Society for General Microbiology Annual Conference 2014, can be viewed via this link: https://www.youtube.com/watch?v=79Z7iXVEPo4
登录
查看更多内容
影响因子：
16
作者：
Kireeva, Maria L.;Nedialkov, Yuri A.;Kashlev, Mikhail
通讯作者：
Kashlev, Mikhail
影响因子：
16
作者：
Zhang, Yan;Mooney, Rachel A.;Grass, Jeffrey A.;Sivaramakrishnan, Priya;Herman, Christophe;Landick, Robert;Wang, Jue D.
通讯作者：
Wang, Jue D.
影响因子：
56.9
作者：
Zaychikov, E;Martin, E;Mustaev, A
通讯作者：
Mustaev, A
影响因子：
64.8
作者：
Tagami, Shunsuke;Sekine, Shun-ichi;Yokoyama, Shigeyuki
通讯作者：
Yokoyama, Shigeyuki
影响因子：
16
作者：
Adelman, K;Yuzenkova, J;Severinov, K
通讯作者：
Severinov, K