PELDOR in rotationally symmetric homo-oligomers.
PELDOR in rotationally symmetric homo-oligomers.
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旋转对称的同性恋者中的Peldor。
DOI：
10.1080/00268976.2013.798697
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发表时间：
2013-10
影响因子：
1.7
通讯作者：
Bode BE
中科院分区：
文献类型：
--
作者：
Giannoulis A;Ward R;Branigan E;Naismith JH;Bode BE
脉冲电子-电子双共振（PELDOR）光谱法测量纳米距离已成为结构生物学中越来越重要的工具。该实验的理论基础是明确的系统含有两个氮氧自旋标记（自旋对），然而，最近的实验已经报道了同源寡聚膜蛋白组成的多达8个自旋标记的单体。我们已经探索了这些系统背后的理论，通过检查模型系统的基础上排列在旋转对称多边形的多个自旋。结果表明，随着测试分子内自旋数的增加，从基于简单自旋对方法的分析获得的距离分布中出现越来越强的扭曲。这些失真在系统尺寸的范围内是显著的，并且即使在模型的对称性中引入随机误差时仍然如此。我们提出了一种替代方法来提取这样的系统上的距离的基础上，适当对待多自旋相关性的最小化。我们证明了这种方法的实用性的自旋标记突变的七聚体机械敏感通道的小电导的E。杆菌
Nanometre distance measurements by pulsed electron–electron double resonance (PELDOR) spectroscopy have become an increasingly important tool in structural biology. The theoretical underpinning of the experiment is well defined for systems containing two nitroxide spin-labels (spin pairs); however, recently experiments have been reported on homo-oligomeric membrane proteins consisting of up to eight spin-labelled monomers. We have explored the theory behind these systems by examining model systems based on multiple spins arranged in rotationally symmetric polygons. The results demonstrate that with a rising number of spins within the test molecule, increasingly strong distortions appear in distance distributions obtained from an analysis based on the simple spin pair approach. These distortions are significant over a range of system sizes and remain so even when random errors are introduced into the symmetry of the model. We present an alternative approach to the extraction of distances on such systems based on a minimisation that properly treats multi-spin correlations. We demonstrate the utility of this approach on a spin-labelled mutant of the heptameric Mechanosensitive Channel of Small Conductance of E. coli.
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影响因子：
15
作者：
Endeward, Burkhard;Butterwick, Joel A.;MacKinnon, Roderick;Prisner, Thomas F.
通讯作者：
Prisner, Thomas F.
影响因子：
1
作者：
Hagelueken, Gregor;Ward, Richard;Naismith, James H.;Schiemann, Olav
通讯作者：
Schiemann, Olav
DOI：
10.1073/pnas.0908050107
发表时间：
2010-02-02
影响因子：
11.1
作者：
Roessler, Maxie M.;King, Martin S.;Hirst, Judy
通讯作者：
Hirst, Judy
影响因子：
15
作者：
Elsässer, C;Brecht, M;Bittl, R
通讯作者：
Bittl, R
影响因子：
3.3
作者：
Jeschke, Gunnar;Sajid, Muhammad;Godt, Adelheid
通讯作者：
Godt, Adelheid