Icariin ameliorates streptozotocin-induced diabetic retinopathy in vitro and in vivo.
Icariin ameliorates streptozotocin-induced diabetic retinopathy in vitro and in vivo.
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淫羊藿苷在体外和体内均可改善链脲佐菌素诱发的糖尿病视网膜病变
DOI：
10.3390/ijms13010866
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发表时间：
2012
影响因子：
5.6
通讯作者：
Xin ZC
中科院分区：
文献类型：
--
作者：
Xin H;Zhou F;Liu T;Li GY;Liu J;Gao ZZ;Bai GY;Lu H;Xin ZC
本研究在链脲佐菌素（STZ）诱导的糖尿病大鼠模型系统中观察了补充淫羊藿苷（伊卡）对糖尿病视网膜病变（DR）的影响。将50只Sprague道利大鼠随机分为对照组和链脲佐菌素诱导的糖尿病组。糖尿病大鼠随机分为两组，一组灌胃伊卡5 mg/kg/d，连续12周，另一组灌胃生理盐水作为安慰剂。视网膜形态学变化，内皮标志物（RECA），胶原IV（Col-IV），血管内皮生长因子（VEGF），和视网膜神经节细胞（RGCs）的神经病变的变化（Thy-1和Brn 3a的表达）进行了研究。还研究了不同浓度（0、101、102、103 nmol/mL）的伊卡对糖尿病和正常动物视网膜神经节细胞（RGC）突起生长的影响。在糖尿病大鼠的视网膜血管中观察到许多病理学变化（RECA、VEGF、Thy-1和Brn 3a表达下降以及IV型胶原和Müller细胞含量降低）;这些变化在接受伊卡的糖尿病动物中减弱。伊卡以剂量依赖方式促进正常大鼠和糖尿病大鼠RGC神经突起生长。伊卡可用于治疗糖尿病视网膜病变。进一步的调查表明。
This study investigated the effect of Icariin (ICA) supplementation on diabetic retinopathy (DR) in a streptozotocin-induced diabetic rat model system. Fifty Sprague Dawley rats were randomly distributed into a control group and a streptozotocin-induced diabetes group. Diabetic rats were randomly divided into two groups; one group received ICA 5 mg/kg/day for 12 weeks by oral gavage; the other group received saline gavage as a placebo. Retinal morphological changes, endothelial markers (RECA), collagen IV (Col-IV), vascular endothelial growth factor (VEGF), and neuropathic changes (Thy-1 and Brn3a expression) of the retinal ganglion cells (RGCs) were investigated. The effects of ICA at various concentrations (0, 101, 102, 103 nmol/mL) on neurite growth were investigated also in retinal ganglion cells (RGC) cultured from both diabetic and normal animals. Numerous pathological changes (deceased expression of RECA, VEGF, Thy-1, and Brn3a as well as decreased Collagen IV and Müller cell content) were noted in the retinal vessels of diabetic rats; these changes were attenuated in diabetic animals that received ICA. ICA enhanced neurite growth in RGC from both normal rats and diabetic rats in a dose dependent fashion. ICA may be useful in the treatment of diabetic retinopathy. Further investigations are indicated.
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影响因子：
3.7
作者：
Jonas, Jost B
通讯作者：
Jonas, Jost B
影响因子：
--
作者：
Chen, Yun;Yang, Rong;Dai, Yutian
通讯作者：
Dai, Yutian
影响因子：
3.7
作者：
Li, Qiangxiang;Ao, Xiang;Wen, Gebo
通讯作者：
Wen, Gebo
影响因子：
16.2
作者：
KLEIN, R;KLEIN, BEK;MOSS, SE
通讯作者：
MOSS, SE
影响因子：
3.4
作者：
Bian, Zong-Mei;Elner, Susan G.;Elner, Victor M.
通讯作者：
Elner, Victor M.