Heterochronic expression of an adult muscle actin gene during ascidian larval development.
Heterochronic expression of an adult muscle actin gene during ascidian larval development.
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海鞘幼虫发育过程中成体肌肉肌动蛋白基因的异时表达。
DOI：
10.1002/dvg.1020150107
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Jeffery,WR
中科院分区：
文献类型：
--
作者：
Swalla,BJ;White,ME;Zhou,J;Jeffery,WR
成虫是一种异时性的发育模式，其中成虫组织和器官在幼虫阶段提前分化。我们研究了海鞘Molgula citrina 成体过程中成体肌肉肌动蛋白基因的表达。海鞘含有多种肌肉肌动蛋白基因，这些基因在幼虫、成虫或生命周期的两个阶段中表达。在具有常规幼虫发育的海鞘物种中，幼虫间充质细胞（被认为是成体中胚层的祖细胞）保持未分化并且不表达肌动蛋白基因。英米。 citrina 中，间充质细胞在幼虫发育过程中提前分化，表明其在成虫过程中发挥着作用。来自 M. 的成年肌肉肌动蛋白基因。 citrin是通过用含有SpMA1（褶皱海鞘成体肌肌动蛋白基因）编码区的探针筛选外套膜cDNA文库而获得的。筛选产生了命名为McMA1的cDNA克隆，其实际上包含肌肉肌动蛋白基因的完整编码区和3'非编码区。推导的McMA1和SpMA1蛋白在氨基酸序列上表现出97％的同一性并且可能由同源基因编码。 McMAl基因在幼虫和成虫中表达，但在幼虫尾部肌肉细胞中不表达，表明它是成虫肌肉肌动蛋白基因。使用3'非编码区探针的原位杂交来确定McMAl基因是否在M.M.的成虫过程中表达。柑橘。 McMAlmRNA首先在尾芽后期阶段仅在间充质细胞中检测到，并在这些细胞迁移到孵化幼虫的未来体腔和心脏原基期间继续在这些细胞中积累。幼虫变态后，McMAl 转录物持续存在于间充质细胞中。结论是，成虫肌肉肌动蛋白基因在成虫过程中表现出异时性表达到幼虫期的转变。柑橘。
Adultation is a hetercchronic mode of development in which adult tissues and organs differentiate precociously during the larval phase. We have investigated the expression of an adult muscle actin gene during adultation in the ascidianMolgula citrina. Ascidians contain multiple muscle actin genes which are expressed in the larva, the adult, or during both phases of the life cycle. In ascidian species with conventional larval development, the larval mesenchyme cells, which are believed to be progenitors of the adult mesoderm, remain undifferentiated and do not express the muscle actin genes. InM. citrina, the mesen‐chyme cells differentiate precociously during larval development, suggesting a role in adultation. An adult muscle actin gene fromM. citrinawas obtained by screening a mantle cDNA library with a probe containing the coding region ofSpMAl, aStyela plicataadult muscle actin gene. The screen yielded a cDNA clone designatedMcMAl, which contained virtually the complete coding and 3′ noncoding regions of a muscle actin gene. The deduced McMAl and SpMAl proteins exhibit 97% identity in amino acid sequence and may be encoded by homologous genes. TheMcMAlgene is expressed in juveniles and adults, but not in larval tail muscle cells, suggesting that it is an adult muscle actin gene. In situ hybridization with a 3′ non‐coding region probe was used to determine whether theMcMAlgene is expressed during adultation inM. citrina. McMAlmRNA was first detected exclusively in the mesenchyme cells during the late tailbud stage and continued to accumulate in these cells during their migration into the future body cavity and heart primordium in the hatched larva. TheMcMAltranscripts persisted in mesenchyme cells after larval metamorphosis. It is concluded that an adult muscle actin gene shows a heterochronic shift of expression into the larval phase during adultation inM. citrina.
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被囊动物 Clavelina picta 中血细胞的胚胎起源。
DOI：
10.2307/3224227
发表时间：
1968
期刊：
Transactions of the American Microscopical Society
影响因子：
--
作者：
R. R. Cowden
通讯作者：
R. R. Cowden
海鞘胚胎肌肉细胞谱系中肌肉肌动蛋白基因的差异表达。
DOI：
10.1242/dev.101.4.751
发表时间：
1987
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Tomlinson,CR;Beach,RL;Jeffery,WR
通讯作者：
Jeffery,WR
Molgula citrina（桤木和汉考克）自由游动幼虫的活动和结构。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
C. Grave
通讯作者：
C. Grave
影响因子：
56.9
作者：
AMBROS, V;HORVITZ, HR
通讯作者：
HORVITZ, HR
通过细胞内注射示踪酶对海鞘胚胎进行细胞谱系分析。
影响因子：
2.7
作者：
Hiroki Nishida;Hiroki Nishida;Noriyuki Satoh;Noriyuki Satoh
通讯作者：
Noriyuki Satoh