Single genome amplification and standard bulk PCR yield HIV-1 envelope products with similar genotypic and phenotypic characteristics.
Single genome amplification and standard bulk PCR yield HIV-1 envelope products with similar genotypic and phenotypic characteristics.
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单基因组扩增和标准散装PCR产生具有相似基因型和表型特征的HIV-1包膜产物。
DOI：
10.1016/j.jviromet.2015.01.006
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发表时间：
2015-03
影响因子：
3.1
通讯作者：
Sagar M
中科院分区：
文献类型：
--
作者：
Etemad B;Ghulam-Smith M;Gonzalez O;White LF;Sagar M
Recent studies suggest that single genome amplification (SGA) as compared to standard bulk PCR and virus stocks from 293T transfection versus short term passage in peripheral blood mononuclear cells (PBMC) yield a less biased representation of HIV-1 envelope characteristics. In 9 different subjects, genetic diversity, divergence, and population structure was not significantly different among SGA or bulk PCR amplified envelope V1–V3 segments. In these subjects, 293T transfection derived virus stocks with SGA or bulk PCR amplified envelopes had similar infectivity, replication kinetics, co-receptor usage, and neutralization susceptibility. While PBMC passage as compared to the 293T derived virus stocks had similar co-receptor usage, PBMC viruses were less neutralization susceptible to some specific antibodies. Our results suggest that the method of envelope sequence amplification, either SGA or bulk PCR, does not have a significant impact on the genotypic and phenotypic properties of the virus envelope quasispecies.
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影响因子：
64.8
作者：
Huang J;Ofek G;Laub L;Louder MK;Doria-Rose NA;Longo NS;Imamichi H;Bailer RT;Chakrabarti B;Sharma SK;Alam SM;Wang T;Yang Y;Zhang B;Migueles SA;Wyatt R;Haynes BF;Kwong PD;Mascola JR;Connors M
通讯作者：
Connors M
影响因子：
56.9
作者：
COFFIN, JM
通讯作者：
COFFIN, JM
影响因子：
2.7
作者：
Dudley, Dawn M.;Gao, Yong;Arts, Eric J.
通讯作者：
Arts, Eric J.
影响因子：
5.4
作者：
Sagar, Manish;Wu, Xueling;Overbaugh, Julie
通讯作者：
Overbaugh, Julie
DOI：
10.1074/jbc.m109.085472
发表时间：
2010-07-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Raska M;Takahashi K;Czernekova L;Zachova K;Hall S;Moldoveanu Z;Elliott MC;Wilson L;Brown R;Jancova D;Barnes S;Vrbkova J;Tomana M;Smith PD;Mestecky J;Renfrow MB;Novak J
通讯作者：
Novak J