Increased Expression of TXNIP Facilitates Oxidative Stress in Nasal Epithelial Cells of Patients With Chronic Rhinosinusitis With Nasal Polyps
Increased Expression of TXNIP Facilitates Oxidative Stress in Nasal Epithelial Cells of Patients With Chronic Rhinosinusitis With Nasal Polyps
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TXNIP 表达增加促进慢性鼻窦炎伴鼻息肉患者鼻上皮细胞的氧化应激
DOI：
10.1177/1945892420982411
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Weitian Zhang
中科院分区：
文献类型：
--
作者：
Hai Lin;Guangyi Ba;Ru Tang;Mingxian Li;Zhipeng Li;Dawei Li;Haibo Ye;Weitian Zhang
氧化应激在慢性鼻窦炎伴鼻息肉（CRSwNP）的发病机制中起重要作用。硫氧还蛋白相互作用蛋白（TXNIP）在触发氧化应激过程中是必不可少的。然而，其在CRSwNP中的作用和机制尚不清楚。本研究旨在探讨TXNIP在CRSwNP发病机制中的作用和机制。方法采用Western blotting、real-time PCR和免疫组化（IHC）检测CRSwNP患者和对照组鼻组织中TXNIP、硫氧还蛋白（TRX）的表达。采用MDA和SOD Assay Kit检测鼻腔组织匀浆中MDA水平和SOD活性。为了评估TXNIP在CRSwNP中的作用和机制，我们用TXNIP siRNA培养和刺激人鼻上皮细胞（HNECs），并添加或不添加n -乙酰半胱氨酸（NAC，一种ROS清除剂）。采用Western blotting、real-time PCR、ROS检测染料DCFH-DA、MDA和SOD Assay Kit检测刺激剂对细胞的作用及机制。结果与对照组相比，CRSwNP患者TXNIP水平升高，TRX蛋白、mRNA、阳性细胞水平降低，MDA水平升高，SOD活性降低。在体外研究中，用或不加NAC的TXNIP siRNA处理HNECs后，TXNIP、TRX、MDA、SOD和ROS水平均发生显著变化。结论在蛋白和mRNA水平上，CRSwNP中TXNIP表达升高，TRX表达降低。CRSwNP组MDA水平升高，SOD活性降低。TXNIP可能与TRX负相关，进而降低SOD活性，升高MDA水平，导致HNECs中ROS和氧化应激水平上调，这可能在CRSwNP的发病机制中起关键作用。未来的研究有望进一步探索TXNIP在CRSwNP中的作用和机制。
Background Oxidative stress plays crucial roles in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). Thioredoxin-interacting protein (TXNIP) is essential in the process of triggering oxidative stress. However, its role and mechanism in CRSwNP remain unclear. The present study sought to explore the role and mechanism of TXNIP in the pathogenesis of CRSwNP. Methods Western blotting, real-time PCR and immunohistochemistry (IHC) were employed to assess TXNIP, thioredoxin (TRX) expression in nasal tissue samples from patients with CRSwNP and control subjects. MDA level and SOD activity in nasal tissue homogenates were measured using MDA and SOD Assay Kit. To evaluate the role and mechanism of TXNIP in CRSwNP, human nasal epithelial cells (HNECs) were cultured and stimulated using TXNIP siRNA, with or without N-acetylcysteine (NAC, an ROS scavenger). Western blotting, real-time PCR, ROS detecting dye DCFH-DA, MDA and SOD Assay Kit were performed to assess the effects and mechanisms of stimulators on the cells. Results We found significantly increased levels of TXNIP and decreased levels of TRX protein, mRNA, positive cells, increased MDA level and decreased SOD activity in CRSwNP patients compared with control subjects. In vitro study, significantly altered levels of TXNIP, TRX, MDA, SOD and ROS in HNECs were found following treatment of TXNIP siRNA with or without NAC on HNECs. Conclusion TXNIP expression was increased and TRX expression was decreased in CRSwNP at both protein and mRNA levels. MDA levels were increased and SOD activities were decreased in CRSwNP. TXNIP may have negative association with TRX, and then decrease SOD activities and increase MDA levels, resulting in the upregulation of ROS and oxidative stress in HNECs, which may play a pivotal role in the pathogenesis of CRSwNP. Future studies are expected to further explore the role and mechanism of TXNIP in CRSwNP.
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影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Wormald, Peter John
通讯作者：
Wormald, Peter John
影响因子：
3.2
作者：
Alhawiti NM;Al Mahri S;Aziz MA;Malik SS;Mohammad S
通讯作者：
Mohammad S
影响因子：
5.5
作者：
Avdeeva K;Fokkens W
通讯作者：
Fokkens W
影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Wormald, Peter John
通讯作者：
Wormald, Peter John
DOI：
10.1016/j.jaci.2015.05.024
发表时间：
2015-12
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Seshadri S;Lu X;Purkey MR;Homma T;Choi AW;Carter R;Suh L;Norton J;Harris KE;Conley DB;Kato A;Avila PC;Czarnocka B;Kopp PA;Peters AT;Grammer LC;Chandra RK;Tan BK;Liu Z;Kern RC;Schleimer RP
通讯作者：
Schleimer RP