Single-cell RNA-seq: advances and future challenges.
Single-cell RNA-seq: advances and future challenges.
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DOI：
10.1093/nar/gku555
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发表时间：
2014-08
影响因子：
14.9
通讯作者：
Vogel J
中科院分区：
文献类型：
--
作者：
Saliba AE;Westermann AJ;Gorski SA;Vogel J
哲学上相同的细胞在其寿命期间可能会大大变化，并且这种变化反映在其分子组成（例如转录景观）中，使用下一代转录本（RNA-SEQ）作为一种强大的工具出现。在基因组量表上的细胞对细胞变异性已经极大地影响了我们对不同的生物学过程的概念性理解，并对基本和临床研究产生了广泛的影响。在这里 - 以自己的优势和当前的限制。蓬勃发展的领域。
Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here—each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.
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