Comparison of Wide-Field/Deconvolution and Confocal Microscopy for 3D Imaging
Comparison of Wide-Field/Deconvolution and Confocal Microscopy for 3D Imaging
复制标题
3D 成像宽视场/反卷积和共焦显微镜的比较
DOI：
10.1007/978-1-4757-5348-6_23
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
P. Shaw
中科院分区：
文献类型：
--
作者：
P. Shaw
光学显微镜一直是生物研究的核心技术，但近年来其重要性大大增加，主要是因为落射荧光成像的引入，它可以提供非常灵敏的检测，再加上多种高度特异性的荧光探针。此外，通常可以在不损害活细胞的光照水平下非侵入性地获得有用的图像。因此，显微注射和其他细胞装载方法可以与荧光显微镜结合使用，以分析和修改活细胞中的亚细胞结构和功能。
Optical microscopy has always been a central technique to biological research, but in recent years its importance has vastly increased, mainly because of the introduction of epifluorescence imaging, which gives very sensitive detection, coupled with a multitude of highly specific fluorescent probes. Furthermore, it is often possible to obtain useful images noninvasively at light levels that are not damaging to living cells. Microinjection and other cell-loading methods can therefore be used in combination with fluorescence microscopy to analyze and modify subcellular structure and function in living cells.
影响因子：
56.9
作者：
HIRAOKA, Y;SEDAT, JW;AGARD, DA
通讯作者：
AGARD, DA