Phospholipase Cγ1 is required for pre-TCR signal transduction and pre-T cell development.
Phospholipase Cγ1 is required for pre-TCR signal transduction and pre-T cell development.
复制标题
DOI：
10.1002/eji.201646522
复制
发表时间：
2017-01
影响因子：
5.4
通讯作者：
Wen R
中科院分区：
文献类型：
--
作者：
Fu G;Yu M;Chen Y;Zheng Y;Zhu W;Newman DK;Wang D;Wen R
Pre-T cell receptor (TCR) signaling is required for pre-T cell survival, proliferation, and differentiation from the CD4 and CD8 double negative (DN) to the double positive (DP) stage. However, the pre-TCR signal transduction pathway is not fully understood and the signaling molecules involved have not been completely identified. Phospholipase Cγ (PLCγ) 1 is an important signaling molecule that generates two second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, that are important to mediate PKC activation and intracellular Ca2+ flux in many signaling pathways. Previously, we have shown that PLCγ1 is important for TCR-mediated signaling, development and T-cell activation, but, the role of PLCγ1 in pre-TCR signal transduction and pre-T cell development is not known. In the current study, we demonstrated that PLCγ1 expression level in pre-T cells was comparable to that in mature T cells. Deletion of PLCγ1 prior to the pre-TCR signaling stage partially blocked the DN3 to DN4 transition and reduced thymic cellularity. We also demonstrated that deletion of PLCγ1 impaired pre-T cell proliferation without affecting cell survival. Further study showed that deficiency of PLCγ1 impaired pre-TCR mediated Ca2+ flux and Erk activation. Thus our studies demonstrate that PLCγ1 is important for pre-TCR mediated signal transduction and pre-T cell development.
登录
查看更多内容
影响因子：
7
作者：
Brock, JH;Mulero, V
通讯作者：
Mulero, V
影响因子：
30.5
作者：
Aifantis, I;Gounari, F;von Boehmer, H
通讯作者：
von Boehmer, H
DOI：
10.1073/pnas.94.18.9797
发表时间：
1997-09-02
影响因子：
11.1
作者：
Cheng, AM;Negishi, I;Pawson, T
通讯作者：
Pawson, T
DOI：
10.1073/pnas.1104295108
发表时间：
2011-07-26
影响因子：
11.1
作者：
Kortum, Robert L.;Sommers, Connie L.;Samelson, Lawrence E.
通讯作者：
Samelson, Lawrence E.
影响因子：
4.4
作者：
Lucas, Julie A.;Felices, Martin;Berg, Leslie J.
通讯作者：
Berg, Leslie J.