Changes in synaptic populations in the spinal dorsal horn following a dorsal rhizotomy in the monkey.
Changes in synaptic populations in the spinal dorsal horn following a dorsal rhizotomy in the monkey.
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DOI：
10.1002/cne.22216
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Ralston HJ 3rd
中科院分区：
文献类型：
--
作者：
Darian-Smith C;Hopkins S;Ralston HJ 3rd
在猴中的研究已经显示在颈背根损伤后的几个月期间大量的神经元重组和行为恢复（DRL; Darian-Smith [2004] J.Comp.Neurol. 470：134-150; Darian-Smith和Ciferri [2005] J.Comp.Neurol. 491：27-45，[2006] J.Comp.Neurol. 498：552-565）。本研究的目的是确定超微结构的突触变化后DRL内的背角（DH）。两只猴子接受单侧DRL，如先前所述（Darian-Smith和Brown [2000] Nat. Neurosci. 3：476-481），其从拇指、食指和中指移除皮肤和本体感受输入。在DRL后4个月终止实验前6周，在体感皮层内绘制了手的电生理表征，并将顺行示踪剂注入重新激活的皮层以标记皮质脊髓末梢。通过脊柱病变区收集切片。皮质脊髓终末和抑制谱分别通过包埋前免疫组织化学和包埋后γ-氨基丁酸（GABA）免疫染色来观察。通过浅表DH系统计数突触元件，包括具有圆形囊泡（R）、多形性扁平囊泡（F;假定抑制性突触）、GABA免疫标记的类似突触（F-GABA）、初级传入突触（C型）、具有致密核心囊泡的突触（D，主要是初级传入）和中间神经元的突触前树突（PSD）的突触轮廓。突触类型通过方差分析进行双边比较。正如预期的那样，我们发现病变侧的C型轮廓显著下降（约为对侧的16%），而R轮廓在双侧没有差异。更令人惊讶的是，损伤侧的F谱（对侧的~170%）和F-GABA谱（对侧的~315%）的数量显著增加。我们的研究结果表明，一个显着增加的抑制回路内的deafferented DH。
Studies in monkeys have shown substantial neuronal reorganization and behavioral recovery during the months following a cervical dorsal root lesion (DRL; Darian-Smith [2004] J. Comp. Neurol. 470:134–150; Darian-Smith and Ciferri [2005] J. Comp. Neurol. 491:27–45, [2006] J. Comp. Neurol. 498:552–565). The goal of the present study was to identify ultrastructural synaptic changes post-DRL within the dorsal horn (DH). Two monkeys received a unilateral DRL, as described previously (Darian-Smith and Brown [2000] Nat. Neurosci. 3:476–481), which removed cutaneous and proprioceptive input from the thumb, index finger, and middle finger. Six weeks before terminating the experiment at 4 post-DRL months, hand representation was mapped electrophysiologically within the somatosensory cortex, and anterograde tracers were injected into reactivated cortex to label corticospinal terminals. Sections were collected through the spinal lesion zone. Corticospinal terminals and inhibitory profiles were visualized by using preembedding immunohistochemistry and postembedding γ-aminobutyric acid (GABA) immunostaining, respectively. Synaptic elements were systematically counted through the superficial DH and included synaptic profiles with round vesicles (R), pleomorphic flattened vesicles (F; presumed inhibitory synapses), similar synapses immunolabeled for GABA (F-GABA), primary afferent synapses (C-type), synapses with dense-cored vesicles (D, mostly primary afferents), and presynaptic dendrites of interneurons (PSD). Synapse types were compared bilaterally via ANOVAs. As expected, we found a significant drop in C-type profiles on the lesioned side (~16% of contralateral), and R profiles did not differ bilaterally. More surprising was a significant increase in the number of F profiles (~170% of contralateral) and F-GABA profiles (~315% of contralateral) on the side of the lesion. Our results demonstrate a striking increase in the inhibitory circuitry within the deafferented DH.
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DOI：
10.1007/bf01175558
发表时间：
1979-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
RALSTON, HJ;RALSTON, DD
通讯作者：
RALSTON, DD
DOI：
10.1073/pnas.89.7.2571
发表时间：
1992-04-01
影响因子：
11.1
作者：
RAUSELL, E;CUSICK, CG;JONES, EG
通讯作者：
JONES, EG
影响因子：
5.3
作者：
Colburn, RW;Rickman, AJ;DeLeo, JA
通讯作者：
DeLeo, JA
影响因子：
2.5
作者：
CHUNG, K;MCNEILL, DL;COGGESHALL, RE
通讯作者：
COGGESHALL, RE
影响因子：
2.8
作者：
Eaton, MJ;Plunkett, JA;Montanez, K
通讯作者：
Montanez, K