Longitudinal profiling of circulating miRNA during cardiac allograft rejection: a proof-of-concept study.
Longitudinal profiling of circulating miRNA during cardiac allograft rejection: a proof-of-concept study.
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心脏异体移植排斥反应期间循环miRNA的纵向分析：一项概念验证研究。
DOI：
10.1002/ehf2.13238
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发表时间：
2021-06
影响因子：
3.8
通讯作者：
Schulze PC
中科院分区：
文献类型：
--
作者：
Kennel PJ;Yahi A;Naka Y;Mancini DM;Marboe CC;Max K;Akat K;Tuschl T;Vasilescu EM;Zorn E;Tatonetti NP;Schulze PC
即使在现代免疫抑制方案的时代，心脏移植（HTx）后的同种异体移植物排斥反应也是一种严重的并发症，并导致HTx后高达三分之一的早期死亡。同种异体移植排斥反应是由一系列免疫机制介导的，导致急性细胞排斥反应（ACR）和/或抗体介导的排斥反应（AMR）。监测同种异体移植排斥反应的金标准是侵入性肌内膜活检，使患者暴露于并发症。关于循环中的miRNAs作为检测心脏移植排斥反应的生物标志物的潜力知之甚少。我们在这里提出了一个系统的分析循环miRNA作为生物标志物和预测移植排斥反应后HTx使用下一代小RNA测序。我们使用下一代小RNA测序来研究HTx接受者（10名健康对照，10名心力衰竭患者，13名ACR和10名AMR）中的循环miRNA。在排斥事件消退之前、期间和之后的不同时间点进行miRNA谱分析。我们发现，与无排斥反应的患者相比，有活检证实的心脏排斥反应的患者血清中有三种miRNA水平显著升高：hsa-miR-139 - 5 p，hsa-miR-151 a-5 p和hsa-miR-186 - 5 p。我们鉴定了可能作为ACR后续发展的潜在预测因子的miRNA：hsa-miR-29 c-3 p（ACR）和hsa-miR-486 - 5 p（AMR）。总体而言，hsa-miR-486 - 5 p与急性排斥反应事件的相关性最强。使用循环中的miRNAs监测心脏移植排斥反应可能是侵入性肌内膜活检的替代策略。
Allograft rejection following heart transplantation (HTx) is a serious complication even in the era of modern immunosuppressive regimens and causes up to a third of early deaths after HTx. Allograft rejection is mediated by a cascade of immune mechanisms leading to acute cellular rejection (ACR) and/or antibody‐mediated rejection (AMR). The gold standard for monitoring allograft rejection is invasive endomyocardial biopsy that exposes patients to complications. Little is known about the potential of circulating miRNAs as biomarkers to detect cardiac allograft rejection. We here present a systematic analysis of circulating miRNAs as biomarkers and predictors for allograft rejection after HTx using next‐generation small RNA sequencing. We used next‐generation small RNA sequencing to investigate circulating miRNAs among HTx recipients (10 healthy controls, 10 heart failure patients, 13 ACR, and 10 AMR). MiRNA profiling was performed at different time points before, during, and after resolution of the rejection episode. We found three miRNAs with significantly increased serum levels in patients with biopsy‐proven cardiac rejection when compared with patients without rejection: hsa‐miR‐139‐5p, hsa‐miR‐151a‐5p, and hsa‐miR‐186‐5p. We identified miRNAs that may serve as potential predictors for the subsequent development of ACR: hsa‐miR‐29c‐3p (ACR) and hsa‐miR‐486‐5p (AMR). Overall, hsa‐miR‐486‐5p was most strongly associated with acute rejection episodes. Monitoring cardiac allograft rejection using circulating miRNAs might represent an alternative strategy to invasive endomyocardial biopsy.
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影响因子：
3.7
作者：
Brown M;Suryawanshi H;Hafner M;Farazi TA;Tuschl T
通讯作者：
Tuschl T
影响因子：
17.1
作者：
De Vlaminck I;Valantine HA;Snyder TM;Strehl C;Cohen G;Luikart H;Neff NF;Okamoto J;Bernstein D;Weisshaar D;Quake SR;Khush KK
通讯作者：
Khush KK
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.