Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector.
Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector.
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Dermacentor variabilis arp2/3复合物及其在节肢动物载体的立克感染中的作用的新颖鉴定。
DOI：
10.1371/journal.pone.0093768
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Macaluso KR
中科院分区：
文献类型：
--
作者：
Petchampai N;Sunyakumthorn P;Guillotte ML;Verhoeve VI;Banajee KH;Kearney MT;Macaluso KR
蜱传斑点热群（SFG）立克次体必须能够感染脊椎动物和节肢动物宿主细胞。宿主肌动蛋白相关蛋白2/3 （Arp2/3）复合物在几种细胞内细菌的侵袭过程和基于肌动蛋白的运动中起重要作用，包括果蝇和哺乳动物细胞中的SFG立克次体。为了研究蜱虫Arp2/3复合体在蜱与立克次体相互作用中的作用，从革螨变异体中鉴定出该蛋白所有亚基的开放阅读框，包括Arp2、Arp3、ARPC1、ARPC2、ARPC3、ARPC4和ARPC5。氨基酸序列分析显示，与黑腹果蝇、小家鼠、智人和酿酒酵母的相应亚基相比，该复合物的同源性在25-88%之间。在变异d (Dv) Arp2和Arp3亚基以及在DvARPC1中观察到的5个推测的WD （Trp-Asp）基序中发现了潜在的ATP结合位点。DvArp2/3复合体所有亚基的转录谱显示，与中肠和唾液腺相比，立克次体感染和未感染的卵巢中mRNA的表达更高。当山田鼠感染蜱子房时，只有DvARPC4 mRNA水平较未感染的组织显著上调。Arp2/3复合体抑制生物实验结果表明，山田鼠入侵蜱组织的能力下降，且在蜱卵巢中差异显著，提示Arp2/3复合体可能与立克次体入侵蜱细胞有关。表征与立克次体感染相关的蜱衍生分子是必要的，以便更好地理解蜱传立克次体疾病的生态学。
Tick-borne spotted fever group (SFG) Rickettsia species must be able to infect both vertebrate and arthropod host cells. The host actin-related protein 2/3 (Arp2/3) complex is important in the invasion process and actin-based motility for several intracellular bacteria, including SFG Rickettsia in Drosophila and mammalian cells. To investigate the role of the tick Arp2/3 complex in tick-Rickettsia interactions, open reading frames of all subunits of the protein including Arp2, Arp3, ARPC1, ARPC2, ARPC3, ARPC4, and ARPC5 were identified from Dermacentor variabilis. Amino acid sequence analysis showed variation (ranging from 25–88%) in percent identity compared to the corresponding subunits of the complex from Drosophila melanogaster, Mus musculus, Homo sapiens, and Saccharomyces cerevisiae. Potential ATP binding sites were identified in D. variabilis (Dv) Arp2 and Arp3 subunits as well as five putative WD (Trp-Asp) motifs which were observed in DvARPC1. Transcriptional profiles of all subunits of the DvArp2/3 complex revealed greater mRNA expression in both Rickettsia-infected and -uninfected ovary compared to midgut and salivary glands. In response to R. montanensis infection of the tick ovary, the mRNA level of only DvARPC4 was significantly upregulated compared to uninfected tissues. Arp2/3 complex inhibition bioassays resulted in a decrease in the ability of R. montanensis to invade tick tissues with a significant difference in the tick ovary, indicating a role for the Arp2/3 complex in rickettsial invasion of tick cells. Characterization of tick-derived molecules associated with rickettsial infection is imperative in order to better comprehend the ecology of tick-borne rickettsial diseases.
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影响因子：
9.8
作者：
Dayel MJ;Mullins RD
通讯作者：
Mullins RD
影响因子：
3.1
作者：
Ceraul, Shane M.;Chung, Ashley;Azad, Abdu F.
通讯作者：
Azad, Abdu F.
影响因子：
3.1
作者：
Hybiske, Kevin;Stephens, Richard S.
通讯作者：
Stephens, Richard S.
DOI：
10.1073/pnas.261419298
发表时间：
2001-12-18
影响因子：
11.1
作者：
Dayel, MJ;Holleran, EA;Mullins, RD
通讯作者：
Mullins, RD
影响因子：
6.7
作者：
Elwell, Cherilyn A.;Ceesay, Alhaji;Kim, Jung Hwa;Kalman, Daniel;Engel, Joanne N.
通讯作者：
Engel, Joanne N.