Tracking Nanometer-Scale Fluorescent Particles in Two Dimensions With a Confocal Microscope
Tracking Nanometer-Scale Fluorescent Particles in Two Dimensions With a Confocal Microscope
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使用共焦显微镜追踪二维纳米级荧光粒子
DOI：
10.1109/tcst.2010.2067449
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发表时间：
2011
影响因子：
4.8
通讯作者：
S. Andersson
中科院分区：
文献类型：
--
作者：
Zhaolong Shen;S. Andersson
介绍了一种在共焦显微镜中跟踪多个纳米级荧光粒子的系统及其实验验证。单个荧光颗粒的位置估计是从在少量离散位置处进行的荧光强度测量生成的。跟踪是通过结合估计过程与线性二次高斯（LQG）调节器。通过将单个粒子的模型组合到单个系统中来跟踪多个粒子，应用相同的LQG框架，然后依次通过每个子系统循环控制。单个和多个粒子的实验结果。出于验证目的，在每个实验期间，使用标准高斯拟合方法捕获并离线分析来自电荷耦合器件相机的图像。估计的轨迹与LQG算法产生的轨迹吻合良好，从而验证了跟踪方案。
A system for tracking multiple nanometer-scale fluorescent particles in a confocal microscope and an experimental validation is described. Position estimates of an individual fluorescent particle are generated from fluorescence intensity measurements taken at a small number of discrete locations. Tracking is achieved by combining the estimation procedure with a linear quadratic Gaussian (LQG) regulator. Multiple particles are tracked by combining the models for individual particles into a single system, applying the same LQG framework, and then cycling the control through each subsystem in turn. Experimental results are presented for single and multiple particles. For validation purposes, during each experiment images from a charge-coupled device camera were captured and analyzed offline using a standard Gaussian fit method. The estimated trajectories were in good agreement with those produced by the LQG algorithm, thereby verifying the tracking scheme.
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影响因子：
3.4
作者：
Thompson, RE;Larson, DR;Webb, WW
通讯作者：
Webb, WW
影响因子：
3.4
作者：
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通讯作者：
VERKMAN, AS
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作者：
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通讯作者：
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作者：
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通讯作者：
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