Neuroinflammation induces glial aromatase expression in the uninjured songbird brain.
Neuroinflammation induces glial aromatase expression in the uninjured songbird brain.
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DOI：
10.1186/1742-2094-8-81
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发表时间：
2011-07-18
影响因子：
9.3
通讯作者：
Saldanha CJ
中科院分区：
文献类型：
--
作者：
Duncan KA;Saldanha CJ
来自外周来源的雌激素以及中枢芳构化在脊椎动物脑中具有神经保护作用。在正常情况下，芳香化酶仅在神经元中表达，然而在缺氧/缺血或机械性脑损伤后;芳香化酶也在星形胶质细胞中发现。这种增加的神经胶质芳构化和随之而来的雌激素合成是神经保护性的，可以促进神经元的存活和修复。虽然雌二醇对神经保护的作用已经得到了很好的研究，但是什么诱导了胶质芳香化酶的表达仍然是未知的。成年雄性斑胸草雀（Taeniopygia guttata）的内皮层被穿透伤。在几个时间点后，使用免疫组织化学检测芳香化酶、IL-1β样和IL-6样的表达。第二组斑马鸟暴露于植物血凝素（PHA），一种炎症剂，直接在端脑的背面，而不会造成穿透性损伤。使用定量实时聚合酶链反应检测mRNA表达和免疫组织化学测定细胞表达来检测芳香化酶、IL-1β样和IL-6样的表达。使用t检验或单因素方差分析，然后使用Tukey Kramers事后检验确定统计学显著性。斑胸草雀脑损伤后，细胞因子的表达先于芳香化酶的表达。这种时间模式表明，细胞因子可能会诱导芳香化酶的表达受损斑胸草雀大脑。此外，诱发以未受伤脑中细胞因子表达增加为特征的神经炎性反应足以诱导神经胶质芳香化酶表达。这些研究是第一次检查机械性脑损伤后鸣禽脑中的神经炎症反应，并描述了一种新的神经免疫信号，以启动胶质细胞中芳香酶的表达。
Estrogens from peripheral sources as well as central aromatization are neuroprotective in the vertebrate brain. Under normal conditions, aromatase is only expressed in neurons, however following anoxic/ischemic or mechanical brain injury; aromatase is also found in astroglia. This increased glial aromatization and the consequent estrogen synthesis is neuroprotective and may promote neuronal survival and repair. While the effects of estradiol on neuroprotection are well studied, what induces glial aromatase expression remains unknown. Adult male zebra finches (Taeniopygia guttata) were given a penetrating injury to the entopallium. At several timepoints later, expression of aromatase, IL-1β-like, and IL-6-like were examined using immunohisotchemistry. A second set of zebra birds were exposed to phytohemagglutinin (PHA), an inflammatory agent, directly on the dorsal surface of the telencephalon without creating a penetrating injury. Expression of aromatase, IL-1β-like, and IL-6-like were examined using both quantitative real-time polymerase chain reaction to examine mRNA expression and immunohistochemistry to determine cellular expression. Statistical significance was determined using t-test or one-way analysis of variance followed by the Tukey Kramers post hoc test. Following injury in the zebra finch brain, cytokine expression occurs prior to aromatase expression. This temporal pattern suggests that cytokines may induce aromatase expression in the damaged zebra finch brain. Furthermore, evoking a neuroinflammatory response characterized by an increase in cytokine expression in the uninjured brain is sufficient to induce glial aromatase expression. These studies are among the first to examine a neuroinflammatory response in the songbird brain following mechanical brain injury and to describe a novel neuroimmune signal to initiate aromatase expression in glia.
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影响因子：
4.4
作者：
Marciano, PG;Eberwine, JH;McIntosh, TK
通讯作者：
McIntosh, TK
影响因子：
6.2
作者：
Cerciat, M.;Unkila, M.;Arevalo, M. -A.
通讯作者：
Arevalo, M. -A.
影响因子：
3.4
作者：
Acarin, L;González, B;Castellano, B
通讯作者：
Castellano, B
DOI：
10.1016/j.jsbmb.2007.09.011
发表时间：
2008-02-01
影响因子：
4.1
作者：
Morissette, M.;Le Saux, M.;Di Paolo, T.
通讯作者：
Di Paolo, T.
DOI：
10.1016/s0169-328x(01)00211-x
发表时间：
2001-09-30
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Allan, SM;Harrison, DC;Rothwell, NJ
通讯作者：
Rothwell, NJ