Tear Proteins Calcium binding protein A4 (S100A4) and Prolactin Induced Protein (PIP) are Potential Biomarkers for Thyroid Eye Disease.
Tear Proteins Calcium binding protein A4 (S100A4) and Prolactin Induced Protein (PIP) are Potential Biomarkers for Thyroid Eye Disease.
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DOI：
10.1038/s41598-018-35096-x
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发表时间：
2018-11-16
影响因子：
4.6
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Chng CL;Seah LL;Yang M;Shen SY;Koh SK;Gao Y;Deng L;Tong L;Beuerman RW;Zhou L
目前还没有可靠的生物标志物来预测自身免疫性甲状腺疾病（AITD）患者的甲状腺眼病（TED）。一些证据支持泪腺参与TED。我们研究的目的是定量地将泪液蛋白谱的变化与TED严重程度的增加联系起来。收集四组患者的泪液样本；AITD无TED （AITD）、AITD伴轻度TED （mild TED）、AITD伴重度TED （severe TED）和正常对照。这项研究共招募了72名患者。在发现阶段，使用相对和绝对定量等压标签（iTRAQ） 4-plex进行定量蛋白质组学分析。为了验证发现阶段的结果，使用所有理论片段离子谱的顺序窗口采集（SWATH）来分析来自正常对照组、AITD、轻度TED和重度TED的独立队列。两个蛋白S100A4和PIP在发现和验证阶段的实验中表现出一致的失调趋势。我们的研究表明，在AITD患者中，不同严重程度和活动程度的泪液蛋白质组存在差异。两种泪液蛋白S100A4和PIP可能作为预测AITD患者进展为严重TED的潜在生物标志物。
There are no reliable biomarkers to predict thyroid eye disease (TED) in patients with autoimmune thyroid disease (AITD) currently. Several evidences support the involvement of the lacrimal gland in TED. The aim of our study was to quantitatively correlate the changes in tear protein profile with increasing severity of TED. Tear samples were collected from four groups of patients; AITD without TED (AITD), AITD with mild TED (mild TED), AITD with severe TED (severe TED) and normal controls. A total of 72 patients were recruited for the study. In discovery phase, isobaric tags for relative and absolute quantification (iTRAQ) 4-plex was used for quantitative proteomics analysis. For verification of results from discovery phase, sequential window acquisition of all theoretical fragment ion spectra (SWATH) was used to analyze an independent cohort from normal controls, AITD, mild TED and severe TED. Two proteins, S100A4 and PIP showed consistent dysregulation trends in the discovery and validation phase experiments. Our study demonstrated the differences in tear proteome across the spectrum of different severity and activity of TED in patients with AITD. Two tear proteins, S100A4 and PIP may serve as potential biomarkers to predict progression to severe TED in patients with AITD.
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