Two- and Three-Dimensional Tracking of MFA2 mRNA Molecules in Mating Yeast.
Two- and Three-Dimensional Tracking of MFA2 mRNA Molecules in Mating Yeast.
复制标题
DOI：
10.3390/cells9102151
复制
发表时间：
2020-09-23
期刊：
影响因子：
6
通讯作者：
Liberman-Aronov S
中科院分区：
文献类型：
--
作者：
Geva P;Komoshvili K;Liberman-Aronov S
细胞内mRNA转运有助于mRNA功能的时空调节和定位翻译。在芽殖酵母（Saccharomyces cerevisiae）中，不对称mRNA转运将约30种特异性mRNA定位于芽尖，包括编码极性和分泌因子的mRNA。基本过程涉及RNA结合蛋白（RBP），分子马达，加工体（PB）和肌动蛋白细胞骨架。最近，发现交配酵母中的信息素a因子的表达依赖于其mRNA的适当定位，MFA 2 mRNA与PB一起聚集在shmoo尖端形成“交配体”，a因子从该交配体中局部表达。确保mRNA正确靶向shmoo尖端的机制知之甚少。在这里，我们分析了MFA2 mRNA运输的动力学和轨迹在生活中，α因子处理的酵母。二维（2D）和三维（3D）分析使我们能够重建颗粒轨迹和估计颗粒速度。利用荧光适配体系统对单个MFA 2 mRNA颗粒进行追踪分析，发现MFA 2 mRNA颗粒的运动有三种类型：振动运动、振荡运动和易位运动。mRNA颗粒的运输是复杂的，一个颗粒可以改变它的运动行为和组成在其旅程的shmoo。加工体组装和肌动蛋白为基础的电机，Myo4p，涉及MFA2 mRNA的运动到shmoo，但都不是必需的，表明多种易位机制在发挥作用。我们的可视化研究提出了在shmoo轴承细胞的定位机制的动态视图。
Intracellular mRNA transport contributes to the spatio-temporal regulation of mRNA function and localized translation. In the budding yeast, Saccharomyces cerevisiae, asymmetric mRNA transport localizes ~30 specific mRNAs including those encoding polarity and secretion factors, to the bud tip. The underlying process involves RNA-binding proteins (RBPs), molecular motors, processing bodies (PBs), and the actin cytoskeleton. Recently, pheromone a-factor expression in mating yeast was discovered to depend on proper localization of its mRNA, MFA2 mRNAs in conjunction with PBs cluster at the shmoo tip to form “mating bodies”, from which a-factor is locally expressed. The mechanism ensuring the correct targeting of mRNA to the shmoo tip is poorly understood. Here we analyzed the kinetics and trajectories of MFA2 mRNA transport in living, alpha-factor treated yeast. Two- (2D) and three-dimensional (3D) analyses allowed us to reconstruct the granule tracks and estimate granule velocities. Tracking analysis of single MFA2 mRNA granules, labeled using a fluorescent aptamer system, demonstrated three types movement: vibrational, oscillatory and translocational. The mRNA granule transport was complex; a granule could change its movement behavior and composition during its journey to the shmoo. Processing body assembly and the actin-based motor, Myo4p, were involved in movement of MFA2 mRNA to the shmoo, but neither was required, indicating that multiple mechanisms for translocation were at play. Our visualization studies present a dynamic view of the localization mechanism in shmoo-bearing cells.
登录
查看更多内容
影响因子：
4.5
作者：
Brodsky, AS;Silver, PA
通讯作者：
Silver, PA
DOI：
10.1083/jcb.200304030
发表时间：
2003-12-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Estrada P;Kim J;Coleman J;Walker L;Dunn B;Takizawa P;Novick P;Ferro-Novick S
通讯作者：
Ferro-Novick S
影响因子：
7.8
作者：
Chung, Sunglan;Takizawa, Peter A.
通讯作者：
Takizawa, Peter A.
影响因子：
64.5
作者：
CHENEY, RE;OSHEA, MK;MOOSEKER, MS
通讯作者：
MOOSEKER, MS
影响因子：
3.5
作者：
Barkai, Eli;Garini, Yuval;Metzler, Ralf
通讯作者：
Metzler, Ralf