Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae.
Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.200304030
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发表时间：
2003-12-22
期刊：
影响因子：
--
通讯作者：
Ferro-Novick S
中科院分区：
文献类型：
--
作者：
Estrada P;Kim J;Coleman J;Walker L;Dunn B;Takizawa P;Novick P;Ferro-Novick S
Myo4p是一种非必需的V型肌球蛋白，对ASH1和IST2 mRNA的芽尖定位是必需的。这些mRNAs通过She2p和She3p蛋白与Myo4p结合。She3p是一种连接Myo4p与其货物的接头蛋白。She2p结合ASH1和IST2 mRNA，而She3p结合She2p和Myo4p。在这里，我们表明Myo4p和She3p，而不是She2p，是萌芽酵母中皮质内质网遗传所必需的。与这一观察结果一致，我们发现皮质内质网遗传不依赖于mRNA的运输。皮质内质网是一种与核膜形成胞质小管连接的动态网络。当药物Latrunculin A(Lat-A)阻断肌动蛋白聚合时，ER小管无法生长。此外，在LAT-A处理和Myo4Δ细胞中观察到细胞质内质小管的数量减少。我们的结果表明，Myo4p和She3p促进内质网小管的生长和定向。
Myo4p is a nonessential type V myosin required for the bud tip localization of ASH1 and IST2 mRNA. These mRNAs associate with Myo4p via the She2p and She3p proteins. She3p is an adaptor protein that links Myo4p to its cargo. She2p binds to ASH1 and IST2 mRNA, while She3p binds to both She2p and Myo4p. Here we show that Myo4p and She3p, but not She2p, are required for the inheritance of cortical ER in the budding yeast Saccharomyces cerevisiae. Consistent with this observation, we find that cortical ER inheritance is independent of mRNA transport. Cortical ER is a dynamic network that forms cytoplasmic tubular connections to the nuclear envelope. ER tubules failed to grow when actin polymerization was blocked with the drug latrunculin A (Lat-A). Additionally, a reduction in the number of cytoplasmic ER tubules was observed in Lat-A–treated and myo4Δ cells. Our results suggest that Myo4p and She3p facilitate the growth and orientation of ER tubules.
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影响因子：
11.4
作者：
NAKAYAMA, K;NAGASU, T;JIGAMI, Y
通讯作者：
JIGAMI, Y
影响因子：
7.8
作者：
FRANZUSOFF, A;REDDING, K;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
56.9
作者：
Long, RM;Singer, RH;Jansen, RP
通讯作者：
Jansen, RP
影响因子：
64.5
作者：
CHENEY, RE;OSHEA, MK;MOOSEKER, MS
通讯作者：
MOOSEKER, MS
影响因子：
3.3
作者：
PREUSS, D;MULHOLLAND, J;BOTSTEIN, D
通讯作者：
BOTSTEIN, D