KIF4 regulates midzone length during cytokinesis.
KIF4 regulates midzone length during cytokinesis.
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DOI：
10.1016/j.cub.2011.04.019
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发表时间：
2011-05-24
期刊：
影响因子：
9.2
通讯作者：
Mitchison, Timothy J.
中科院分区：
文献类型：
--
作者：
Hu, Chi-Kuo;Coughlin, Margaret;Field, Christine M.;Mitchison, Timothy J.
中间区，也称为中央纺锤体，是在动物细胞胞质分裂期间在分离的染色体之间形成的反平行微管阵列。中间区可以被认为是招募特定蛋白质和协调细胞动力学事件的平台，例如保持姐妹核分开，沟内移和分裂。尽管这一重要作用，许多方面的中区生物学仍然未知，包括动态组织的中区微管。研究中间区微管动力学一直很困难，部分原因是它们的正末端相互交叉并埋在致密的基质中，使它们难以观察。我们采用单极胞质分裂显示，中间区加端出现非动态。我们确定的chromokinesin KIF4作为一个负调节中间区加端动力学，其活动控制中间区的长度，但不稳定。KIF4是在后期终止中间区伸长所必需的。在没有KIF4的情况下，中间带异常伸长，并且它们的重叠区域没有聚焦。电子致密物质和中间体都没有从延长的中间区，和肌动蛋白丝从沟皮质不解体后进入。KIF4介导的中间区长度调节似乎通过在胞质分裂期间的特定时间终止中间区伸长而发生，使得中间区和有丝分裂纺锤体在其动力学和长度调节机制上不同。
Midzones, also called central spindles, are an array of antiparallel microtubules that form during animal cell cytokinesis between the separated chromosomes. Midzones can be considered platforms that recruit specific proteins and orchestrate cytokinetic events, such as keeping sister nuclei apart, furrow ingression, and abscission. Despite this important role, many aspects of midzone biology remain unknown, including the dynamic organization of midzone microtubules. Investigating midzone microtubule dynamics has been difficult in part because their plus-ends are interdigitated and buried in a dense matrix, making them difficult to observe. We employed monopolar cytokinesis to reveal that midzone plus-ends appear non-dynamic. We identified the chromokinesin KIF4 as a negative regulator of midzone plus-end dynamics, whose activity controls midzone length, but not stability. KIF4 is required to terminate midzone elongation in late anaphase. In the absence of KIF4, midzones elongate abnormally, and their overlap regions are unfocused. Electron dense material and midbodies are both absent from the elongated midzones, and actin filaments from the furrow cortex are not disassembled after ingression. KIF4 mediated midzone length regulation appears to occur by terminating midzone elongation at a specific time during cytokinesis, making midzones and mitotic spindles differ in their dynamics and length regulating mechanisms.
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DOI：
10.1083/jcb.200511061
发表时间：
2006-01-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gruneberg U;Neef R;Li X;Chan EH;Chalamalasetty RB;Nigg EA;Barr FA
通讯作者：
Barr FA
影响因子：
3.7
作者：
Brennan IM;Peters U;Kapoor TM;Straight AF
通讯作者：
Straight AF
影响因子：
9.2
作者：
Hickson, GRX;Echard, A;O'Farrell, PH
通讯作者：
O'Farrell, PH
影响因子：
9.2
作者：
Goshima, G;Wollman, R;Vale, RD
通讯作者：
Vale, RD
影响因子：
7
作者：
Ozlu, Nurhan;Monigatti, Flavio;Steen, Judith J.
通讯作者：
Steen, Judith J.