Identification of differentially expressed non-coding RNAs in embryonic stem cell neural differentiation.
Identification of differentially expressed non-coding RNAs in embryonic stem cell neural differentiation.
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DOI：
10.1093/nar/gks311
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发表时间：
2012-07
影响因子：
14.9
通讯作者：
Hüttenhofer A
中科院分区：
文献类型：
--
作者：
Skreka K;Schafferer S;Nat IR;Zywicki M;Salti A;Apostolova G;Griehl M;Rederstorff M;Dechant G;Hüttenhofer A
蛋白质编码基因将ES细胞分化为神经细胞，但对于NCRNA的类别而言，蛋白质的基因已广泛研究。非编码RNA（NCRNA）转录组，参与小鼠ES细胞为神经细胞的分化，我们已经产生了三个专门的核心核蛋白粒子（RNP）衍生cDNA文库，即通过高通量测序和转录谱分别从多能ES细胞，神经元祖细胞和分化的神经元细胞。在ES细胞分化过程中，7SL，7SK和VAULT-2 RNA的表达显着上调。因此，新颖的NCRNA候选者的大小为18-30 nt，因此类似于mirna物种，但除了少数例外，缺乏规范的miRNA特征。衍生物，但也在主要海马神经元和星形胶质细胞的培养物，增强其在神经元和神经元细胞分化中的潜在功能。
Protein-coding genes, guiding differentiation of ES cells into neural cells, have extensively been studied in the past. However, for the class of ncRNAs only the involvement of some specific microRNAs (miRNAs) has been described. Thus, to characterize the entire small non-coding RNA (ncRNA) transcriptome, involved in the differentiation of mouse ES cells into neural cells, we have generated three specialized ribonucleo-protein particle (RNP)-derived cDNA libraries, i.e. from pluripotent ES cells, neural progenitors and differentiated neural cells, respectively. By high-throughput sequencing and transcriptional profiling we identified several novel miRNAs to be involved in ES cell differentiation, as well as seven small nucleolar RNAs. In addition, expression of 7SL, 7SK and vault-2 RNAs was significantly up-regulated during ES cell differentiation. About half of ncRNA sequences from the three cDNA libraries mapped to intergenic or intragenic regions, designated as interRNAs and intraRNAs, respectively. Thereby, novel ncRNA candidates exhibited a predominant size of 18–30 nt, thus resembling miRNA species, but, with few exceptions, lacking canonical miRNA features. Additionally, these novel intraRNAs and interRNAs were not only found to be differentially expressed in stem-cell derivatives, but also in primary cultures of hippocampal neurons and astrocytes, strengthening their potential function in neural ES cell differentiation.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
10.5
作者：
Babiarz, Joshua E.;Ruby, J. Graham;Blelloch, Robert
通讯作者：
Blelloch, Robert
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
2.5
作者：
Chen, Caifu;Ridzon, Dana;Strauss, William M.
通讯作者：
Strauss, William M.
DOI：
10.1073/pnas.250426397
发表时间：
2000-12-19
影响因子：
11.1
作者：
Cavaillé, J;Buiting, K;Hüttenhofer, A
通讯作者：
Hüttenhofer, A