Highly Efficient Expression of Proteins Encoded by Recombinant Vaccinia Virus in Lymphocytes
Highly Efficient Expression of Proteins Encoded by Recombinant Vaccinia Virus in Lymphocytes
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重组痘苗病毒编码蛋白在淋巴细胞中高效表达
DOI：
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发表时间：
1991
影响因子：
3.7
通讯作者：
C. Martínez
中科院分区：
文献类型：
--
作者：
J. Alonso;J. Rodríguez;E. Viñuela;G. Kroemer;C. Martínez
使用表达E.大肠杆菌β-半乳糖苷酶（β-Gal）感染淋巴细胞，结果表明，T细胞中β-Gal的表达较B细胞低，VV感染可抑制B细胞的生长，但对T细胞的增殖几乎没有影响。此外，在T淋巴细胞中感染性病毒颗粒的产生不太明显。动力学研究表明，在初始剂量依赖性生长抑制后，T细胞继续增殖，而倍增时间不受VV感染的影响。尽管如此。T细胞确实表达由重组VV编码的蛋白。这种β半乳糖或分泌可溶性蛋白质如白细胞介素-4，尽管在每个细胞水平上的效率低于B淋巴细胞。总之，T细胞的生理学似乎比B细胞更少地受到VV的干扰，尽管病毒能够将重组基因的表达引导至T淋巴细胞。
Using a recombinant vaccinia virus (VV) that expresses E. coli β galactosidase (β‐Gal) to infect lymphocytes, we show that enzymometrically or immunologically detectable β‐Gal expression is less pronounced among T cells than among B cells, VV infection caused growth inhibition of B cells, but barely affected T‐cell proliferation in vitro. Moreover, the production of infectious viral particles was less pronounced in T lymphocytes. Kinetic studies revealed that after an initial dose‐dependent growth inhibition, T cells continued to proliferate without the doubling time being affected by VV infection. Nonetheless. The T cells do express proteins encoded by recombinant VV. such β‐Gal. or secrete soluble proteins such as interleukin‐4, though at a lower efficiency at the per cell level than B lymphocytes. In conclusion, the physiology of T cells appears lo be less perturbed by VV than that of B ceils, although the virus is capable of directing expression of recombinant genes to T lymphocytes.
使用重组痘苗病毒证明了针对 Epstein-Barr 病毒核抗原的人类细胞毒性 T 细胞反应。
DOI：
10.1073/pnas.87.8.2906
发表时间：
1990
影响因子：
11.1
作者：
Murray,RJ;Kurilla,MG;Griffin,HM;Brooks,JM;Mackett,M;Arrand,JR;Rowe,M;Burrows,SR;Moss,DJ;Kieff,E
通讯作者：
Kieff,E
影响因子：
4.6
作者：
Pogo,BG;Lai,AC;Holland,JG;Friend,C
通讯作者：
Friend,C