Theta-frequency selectivity in the somatic spike-triggered average of rat hippocampal pyramidal neurons is dependent on HCN channels.
Theta-frequency selectivity in the somatic spike-triggered average of rat hippocampal pyramidal neurons is dependent on HCN channels.
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DOI：
10.1152/jn.00356.2017
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发表时间：
2017-10-01
影响因子：
2.5
通讯作者：
Narayanan R
中科院分区：
文献类型：
--
作者：
Das A;Narayanan R
我们先前预测，使用计算分析，海马神经元的尖峰触发的平均值（STA）将表现出θ频率（4-10 Hz）的频谱选择性，并将表现出对γ频带（25-150 Hz）输入的重合检测能力。在这里，我们证实了这些预测，通过直接电生理记录STA从大鼠CA 1锥体神经元，并表明，阻断HCN通道降低频率的STA频谱选择性的δ频率范围（0.5-4 Hz）。从传入输入的复杂时空模式中提取特定频率的能力是神经元接收频率复用输入的关键功能要求。虽然θ频率阈下共振的表达建立在海马锥体神经元，它是不知道，如果他们的尖峰启动动力学表现光谱选择性，或者如果他们的内在属性被调谐到处理γ频率输入。在这里，我们测量了尖峰触发的平均值（STA）的大鼠海马锥体神经元通过电生理记录和量化的光谱选择性在其尖峰起始动力学和符合检测窗口（CDW）。我们的研究结果显示，STA，这也被赋予了γ-范围CDW，具有突出的神经元到神经元的变异性，表现出不同的成对解离和相关性与不同的内在测量强θ-频率选择性。此外，我们表明，STA和它的测量基本上适应的状态，由其膜电位和其传入输入的统计定义的神经元。最后，我们测试了阻断超极化激活的环核苷酸门控（HCN）通道对STA的影响，发现阻断HCN通道后STA特征频率显著降低至δ频段。STA中的这种δ频率选择性出现在不存在阈下共振的情况下，阈下共振被HCN通道阻断所废除，从而证实了对这两种形式的光谱选择性之间的解离的计算预测。我们的研究结果扩大了HCN通道的作用，θ-频率选择性的穗启动动力学，除了强调不同的离子通道之间的相互作用，在调节神经元生理的关键作用。我们之前曾预测，使用计算分析，海马神经元的尖峰触发平均值（STA）将表现出θ频率（4-10 Hz）的频谱选择性，并将表现出对伽马频带（25-150 Hz）输入的重合检测能力。在这里，我们证实了这些预测，通过直接电生理记录STA从大鼠CA 1锥体神经元，并表明，阻断HCN通道降低频率的STA频谱选择性的δ频率范围（0.5-4 Hz）。
We had previously predicted, using computational analyses, that the spike-triggered average (STA) of hippocampal neurons would exhibit theta-frequency (4–10 Hz) spectral selectivity and would manifest coincidence detection capabilities for inputs in the gamma-frequency band (25–150 Hz). Here, we confirmed these predictions through direct electrophysiological recordings of STA from rat CA1 pyramidal neurons and demonstrate that blocking HCN channels reduces the frequency of STA spectral selectivity to the delta-frequency range (0.5–4 Hz). The ability to distill specific frequencies from complex spatiotemporal patterns of afferent inputs is a pivotal functional requirement for neurons residing in networks receiving frequency-multiplexed inputs. Although the expression of theta-frequency subthreshold resonance is established in hippocampal pyramidal neurons, it is not known if their spike initiation dynamics manifest spectral selectivity, or if their intrinsic properties are tuned to process gamma-frequency inputs. Here, we measured the spike-triggered average (STA) of rat hippocampal pyramidal neurons through electrophysiological recordings and quantified spectral selectivity in their spike initiation dynamics and their coincidence detection window (CDW). Our results revealed strong theta-frequency selectivity in the STA, which was also endowed with gamma-range CDW, with prominent neuron-to-neuron variability that manifested distinct pairwise dissociations and correlations with different intrinsic measurements. Furthermore, we demonstrate that the STA and its measurements substantially adapted to the state of the neuron defined by its membrane potential and to the statistics of its afferent inputs. Finally, we tested the effect of pharmacologically blocking the hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels on the STA and found that the STA characteristic frequency reduced significantly to the delta-frequency band after HCN channel blockade. This delta-frequency selectivity in the STA emerged in the absence of subthreshold resonance, which was abolished by HCN channel blockade, thereby confirming computational predictions on the dissociation between these two forms of spectral selectivity. Our results expand the roles of HCN channels to theta-frequency selectivity in the spike initiation dynamics, apart from underscoring the critical role of interactions among different ion channels in regulating neuronal physiology. NEW & NOTEWORTHY We had previously predicted, using computational analyses, that the spike-triggered average (STA) of hippocampal neurons would exhibit theta-frequency (4–10 Hz) spectral selectivity and would manifest coincidence detection capabilities for inputs in the gamma-frequency band (25–150 Hz). Here, we confirmed these predictions through direct electrophysiological recordings of STA from rat CA1 pyramidal neurons and demonstrate that blocking HCN channels reduces the frequency of STA spectral selectivity to the delta-frequency range (0.5–4 Hz).
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影响因子：
3.4
作者：
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通讯作者：
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DOI：
10.1073/pnas.142213199
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影响因子：
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