Identification of a glutathione transporter in A. actinomycetemcomitans.
Identification of a glutathione transporter in A. actinomycetemcomitans.
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DOI：
10.1128/spectrum.03511-23
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发表时间：
2024-01-11
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
细菌依靠细胞外的化学信号来感知它们的环境，相互作用，塑造它们的化学景观。了解这种生物化学可以帮助我们阐明细菌相互作用影响群落功能的机制。通过将质谱法应用于口腔病原体放线菌聚合菌和口腔共生链球菌的单培养和共培养，我们鉴定了这些细菌产生的数百种细胞外小分子。我们发现放线菌（a . actinomycetemcomitans）分泌毫摩尔量的谷胱甘肽，并鉴定了一个五基因操纵子（称为gttABCDE），它对最大分泌很重要。本研究生成的代谢组学数据集为揭示单个分子在口腔中介导多微生物相互作用的重要性提供了有价值的数据集。微生物产生大量的细胞外分子，这些分子作为信号和线索促进多微生物相互作用，改变微生物群落的功能。这在人类口腔微生物组中得到了特别充分的研究，其中的关键代谢物已被证明对健康和疾病都有影响。在这里，我们使用非靶向质谱方法来全面评估病原体放线菌聚集菌和共生戈登链球菌在单培养和共培养过程中的细胞外代谢组。我们生成并公开了一个代谢组学数据集，其中包括数百种潜在的代谢物，并利用该数据集确定了a .放线菌comitans中谷胱甘肽分泌的重要操纵子。
Bacteria rely on extracellular chemical cues to sense their environment, interact with one another, and shape their chemical landscape. Understanding this biochemistry can help us elucidate the mechanisms by which bacterial interactions impact community function. By applying mass spectrometry to mono- and co-cultures of the oral pathogen Aggregatibacter actinomycetemcomitans and the oral commensal Streptococcus gordonii, we identified hundreds of extracellular small molecules produced by these bacteria. We discovered that A. actinomycetemcomitans secretes millimolar amounts of glutathione and identified a five-gene operon (called gttABCDE) that is important for maximum secretion. The metabolomics data set generated in this study provides a valuable data set for unraveling the importance of individual molecules for mediating polymicrobial interactions in the oral cavity. Microbes produce a large array of extracellular molecules, which serve as signals and cues to promote polymicrobial interactions and alter the function of microbial communities. This has been particularly well studied in the human oral microbiome, where key metabolites have been shown to impact both health and disease. Here, we used an untargeted mass spectrometry approach to comprehensively assess the extracellular metabolome of the pathogen Aggregatibacter actinomycetemcomitans and the commensal Streptococcus gordonii during mono- and co-culture. We generated and made publicly available a metabolomic data set that includes hundreds of potential metabolites and leveraged this data set to identify an operon important for glutathione secretion in A. actinomycetemcomitans.
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影响因子：
11.4
作者：
Ku JWK;Gan YH
通讯作者：
Gan YH
影响因子：
3.2
作者：
Brown, Stacie A.;Whiteley, Marvin
通讯作者：
Whiteley, Marvin
影响因子：
5.6
作者：
HILES, ID;GALLAGHER, MP;HIGGINS, CF
通讯作者：
HIGGINS, CF
影响因子：
3.7
作者：
Amarasinghe, J. J.;Connell, T. D.;Haase, E. M.
通讯作者：
Haase, E. M.
DOI：
10.1073/pnas.1400586111
发表时间：
2014-05-27
影响因子：
11.1
作者：
Stacy, Apollo;Everett, Jake;Whiteley, Marvin
通讯作者：
Whiteley, Marvin