Effect of norfloxacin and moxifloxacin on melanin synthesis and antioxidant enzymes activity in normal human melanocytes.
Effect of norfloxacin and moxifloxacin on melanin synthesis and antioxidant enzymes activity in normal human melanocytes.
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DOI：
10.1007/s11010-014-2297-7
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发表时间：
2015-03
影响因子：
4.3
通讯作者：
Buszman, Ewa
中科院分区：
文献类型：
--
作者：
Beberok, Artur;Wrzesniok, Dorota;Otreba, Michal;Milinski, Maciej;Rok, Jakub;Buszman, Ewa
氟喹诺酮类抗生素可广泛用于治疗多种传染病，包括呼吸道和泌尿道感染。这些药物的重要副作用之一是光毒性，这严重限制了它们的使用。为了深入了解氟喹诺酮类药物诱导的光毒性副作用的分子机制，测定了具有不同光毒性潜力的两种氟喹诺酮类衍生物诺氟沙星和阿托沙星对正常人黑素细胞HEMa-LP中黑素生成和抗氧化酶活性的影响。两种药物均诱导黑素细胞活力的浓度依赖性损失。发现这些药物的EC 50值为0.5 mM。诺氟沙星和诺氟沙星抑制黑色素的生物合成;抗生素被证明可以抑制细胞酪氨酸酶的活性，减少黑色素细胞中的黑色素含量。当比较两种分析的氟喹诺酮类时，观察到诺氟沙星对黑素细胞中酪氨酸酶活性的抑制作用大于诺氟沙星。通过测量抗氧化酶：SOD、CAT和GPx的活性来评估细胞中氧化应激的程度。据观察，诺氟沙星引起更高的消耗的抗氧化状态的黑素细胞相比，氟沙星。所获得的结果提供了一个新的洞察机制氟喹诺酮类药物毒性针对色素组织。此外，在黑素细胞中的生化过程的调制的差异可能是各种光毒性活性的分析氟喹诺酮衍生物在体内的解释。
Fluoroquinolone antibiotics provide broad-spectrum coverage for a number of infectious diseases, including respiratory as well as urinary tract infections. One of the important adverse effects of these drugs is phototoxicity which introduces a serious limitation to their use. To gain insight the molecular mechanisms underlying the fluoroquinolones-induced phototoxic side effects, the impact of two fluoroquinolone derivatives with different phototoxic potential, norfloxacin and moxifloxacin, on melanogenesis and antioxidant enzymes activity in normal human melanocytes HEMa-LP was determined. Both drugs induced concentration-dependent loss in melanocytes viability. The value of EC50 for these drugs was found to be 0.5 mM. Norfloxacin and moxifloxacin suppressed melanin biosynthesis; antibiotics were shown to inhibit cellular tyrosinase activity and to reduce melanin content in melanocytes. When comparing the both analyzed fluoroquinolones, it was observed that norfloxacin possesses greater inhibitory effect on tyrosinase activity in melanocytes than moxifloxacin. The extent of oxidative stress in cells was assessed by measuring the activity of antioxidant enzymes: SOD, CAT, and GPx. It was observed that norfloxacin caused higher depletion of antioxidant status in melanocytes when compared with moxifloxacin. The obtained results give a new insight into the mechanisms of fluoroquinolones toxicity directed to pigmented tissues. Moreover, the presented differences in modulation of biochemical processes in melanocytes may be an explanation for various phototoxic activities of the analyzed fluoroquinolone derivatives in vivo.
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影响因子：
3.6
作者：
Plonka PM;Passeron T;Brenner M;Tobin DJ;Shibahara S;Thomas A;Slominski A;Kadekaro AL;Hershkovitz D;Peters E;Nordlund JJ;Abdel-Malek Z;Takeda K;Paus R;Ortonne JP;Hearing VJ;Schallreuter KU
通讯作者：
Schallreuter KU
影响因子：
2.8
作者：
Beberok, Artur;Zdybel, Magdalena;Wrzesniok, Dorota
通讯作者：
Wrzesniok, Dorota
DOI：
10.1111/j.1600-0749.1993.tb00591.x
发表时间：
1993-06-01
期刊：
PIGMENT CELL RESEARCH
影响因子：
--
作者：
LARSSON, BS
通讯作者：
LARSSON, BS
影响因子：
1.6
作者：
Buszman, E.;Beberok, A.;Orzechowska, A.
通讯作者：
Orzechowska, A.
影响因子：
2
作者：
Kim, DS;Kim, SY;Park, KC
通讯作者：
Park, KC