Ultrastructure of the basal lamina and its relationship to extracellular matrix of embryos of the starfish Pisaster ochraceus as revealed by anionic dyes
Ultrastructure of the basal lamina and its relationship to extracellular matrix of embryos of the starfish Pisaster ochraceus as revealed by anionic dyes
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阴离子染料揭示海星胚胎基底层超微结构及其与细胞外基质的关系
DOI：
10.1002/jmor.1051990309
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发表时间：
1989
影响因子：
1.5
通讯作者：
B. Crawford
中科院分区：
文献类型：
--
作者：
B. Crawford
通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）以及阴离子染料处理后，对51/2-6天胚胎小行星基底层（BL）的超微结构进行了研究。传统的固定在戊二醛和锇揭示了一个BL组成的致密层从基底细胞表面分离的一个层。很少或没有网状层存在。与基底层外观相似的物质延伸到囊胚腔中，形成细胞外基质（ECM）。在钌红染料存在下固定后，在透明板和致密板下方可见蛋白聚糖（PG）颗粒。ECM由类似外观的颗粒组成，其与类似于无脊椎动物胶原的中等电子密度的纤维相关。在聚阴离子条件下，在阿辛蓝存在下固定后，基底层和ECM的所有方面都染色非常密集。在0.3 M MgCl 2（单阴离子条件）或低浓度下使用阿辛蓝，显示出由精细毡织和小管样结构组成的致密层。外胚层致密层的内部（靠近囊胚腔）嵌入一个由厚的、染色密集的和薄的、染色中等的纤维束组成的网状结构。内胚层BL中存在类似的元素，但致密材料由不形成网状结构的短区域表示。两个基底层的致密股和中间股也延伸到囊胚腔作为ECM。小管样结构从内网的致密材料延伸到致密层。它们也穿过致密层和透明层与基底细胞膜结合。基底细胞表面经常在接触点处向外起皱的事实表明，这种构造可能提供了一种手段，由此力可以从ECM通过基底层转移到细胞。
The ultrastructure of the 51/2–6‐day‐old embryonic asteroid basal lamina (BL) was studied by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and after treatment with anionic dyes. Conventional fixation in glutaraldehyde and osmium reveals a BL consisting of a lamina densa separated from the basal cell surface by a lamina lucida. Little or no reticular lamina is present. Material similar in appearance to the basal lamina extends into the blastocoel, forming an extracellular matrix (ECM). Following fixation in the presence of the dye ruthenium red, proteoglycan (PG) granules are visible in the lamina lucida and immediately beneath the lamina densa. The ECM consists of granules of a similar appearance, which are associated with fibers of an intermediate electron density resembling invertebrate collagen. After fixation in the presence of alcian blue under polyanionic conditions, all aspects of the basal lamina and the ECM stain very densely. The use of alcian blue in 0.3 M MgCl2 (monoanionic condition) or in low concentrations reveals a lamina densa consisting of a fine feltwork and tubule‐like structures. A meshwork composed of thick, densely stained and thinner, intermediately stained strands is embedded in the inner aspect (that adjacent to the blastocoel) of the ectodermal lamina densa. Similar elements are present in the endodermal BL, but the dense material is represented by short regions that do not form a meshwork. The dense and intermediate strands of both basal laminae also extend into the blastocoel as ECM. The tubule‐like structures extend from the dense material of the inner meshwork into the lamina densa. They also cross both the lamina densa and lucida to associatee with the basal cell membranes. The fact that the basal cell surfaces are often puckered outward at the points of contact suggests that this configuration might be providing a means whereby forces can be transferred from the ECM through the basal lamina to the cells.
海胆胚胎中的原肠胚形成需要交联胶原蛋白在细胞外基质内的沉积。
影响因子：
2.7
作者：
Wessel,GM;McClay,DR
通讯作者：
McClay,DR
海胆胚胎基底层的个体发育。
影响因子：
2.7
作者：
Wessel,GM;Marchase,RB;McClay,DR
通讯作者：
McClay,DR