Ontogeny of the basal lamina in the sea urchin embryo.

Ontogeny of the basal lamina in the sea urchin embryo.
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海胆胚胎基底层的个体发育。

DOI:
10.1016/0012-1606(84)90025-3
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发表时间:
1984
影响因子:
2.7
通讯作者:
McClay,DR
McClay,DR
中科院分区:
生物学3区
文献类型:
--
作者:
Wessel,GM;Marchase,RB;McClay,DR

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海胆胚胎发育过程中的几个细胞外基质成分的表达模式进行了描述。在石蜡切片的材料上采用免疫荧光测定,使用(i)针对已知脊椎动物细胞外基质组分的多克隆抗体:层粘连蛋白、纤连蛋白、硫酸乙酰肝素蛋白聚糖、I型、III型和IV型胶原蛋白;和(ii)针对海胆胚胎组分产生的单克隆抗体。研究发现,大多数细胞外基质成分位于未受精卵中的颗粒(0.5-2.0 μm)中,与皮质颗粒不同。受精启动了细胞外基质(ECM)成分从卵颗粒内运输到发育胚胎的基底层。各种ECM成分在不同的时间到达发育中的基底层,并且并非所有成分都是基底层所特有的。两种ECM成分在卵内未发现。这些分子在间充质囊胚期才出现,并在整个发育过程中对中胚层保持特异性。抗体对脊椎动物ECM抗原与海胆胚胎组分的反应性表明门之间存在免疫学相似的ECM分子。
The patterns of expression for several extracellular matrix components during development of the sea urchin embryo are described. An immunofluorescence assay was employed on paraffin-sectioned material using (i) polyclonal antibodies against known vertebrate extracellular matrix components: laminin, fibronectin, heparan sulfate proteoglycan, collagen types I, III, and IV; and (ii) monoclonal antibodies generated against sea urchin embryonic components. Most extracellular matrix components studied were found localized within the unfertilized egg in granules (0.5–2.0 μm) distinct from the cortical granules. Fertilization initiated trafficking of the extracellular matrix (ECM) components from within the egg granules to the basal lamina of the developing embryo. The various ECM components arrived within the developing basal lamina at different times, and not all components were unique to the basal lamina. Two ECM components were not found within the egg. These molecules appearedde novoat the mesenchyme blastula stage, and remained specific to the mesoderm through development. The reactivity of antibodies to vertebrate ECM antigens with components of the sea urchin embryo suggests the presence of immunologically similar ECM molecules between the phyla.
基底膜(IV 型)胶原蛋白的免疫化学研究。
DOI: --
发表时间: 1979
期刊: Immunology
影响因子: 6.4
作者:
R. Timpl;R. Glanville;G. Wick;G. Martin
通讯作者: G. Martin
海胆发育过程中脯氨酰羟化酶活性。
DOI: 10.1016/0014-4827(80)90028-2
发表时间: 1980
影响因子: 3.7
作者:
S. Benson;A. Sessions
通讯作者: A. Sessions
硫酸盐在海胆(Paracentrotus lividus)胚胎分化中的作用分析
DOI: 10.5962/bhl.part.75596
发表时间: 1964
影响因子: 0.8
作者:
J. Runnström;S. Hörstadius;J. Immers;M. Fudge
通讯作者: M. Fudge
放线菌 D 对细胞成分和乌尔辛卵细胞 (Paracentrotus lividus) 代谢的影响:放射自显影研究
DOI: 10.1016/0014-4827(68)90389-3
发表时间: 1968
影响因子: 3.7
作者:
S. Sanchez
通讯作者: S. Sanchez
透明层是海胆胚胎和重新聚集细胞中含有胶原蛋白的细胞外基质。
DOI: 10.1016/0014-4827(79)90495-6
发表时间: 1979
影响因子: 3.7
作者:
E. Spiegel;M. Spiegel
通讯作者: M. Spiegel