Photophysical properties of Cerulean and Venus fluorescent proteins.
Photophysical properties of Cerulean and Venus fluorescent proteins.
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DOI：
10.1117/1.3156842
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发表时间：
2009-05
影响因子：
3.5
通讯作者：
Gryczynski Z
中科院分区：
文献类型：
--
作者：
Sarkar P;Koushik SV;Vogel SS;Gryczynski I;Gryczynski Z
Cerulean和Venus是最近开发的荧光蛋白，经常被研究人员用作基于FRET的共定位研究中的供体-受体对。我们表征了这两种蛋白质在宽光谱范围内（从紫外到可见光区域）的荧光特性。激发光谱，寿命和偏振光谱显示显着的能量转移芳香族氨基酸的荧光蛋白生色团。高的稳态各向异性值和各向异性衰减中快速分量的缺乏表明荧光蛋白发色团被刚性地固定在蛋白质结构内。此外，我们表明，发色团是不可访问的外部淬灭剂，如丙烯酰胺或碘化钾（KI），允许去除“不需要的”背景的环境中与外部淬灭剂，而留下的Cerulean/金星荧光不变。
Cerulean and Venus are recently developed fluorescent proteins, often used as a donor-acceptor pair by researchers in FRET based co-localization studies. We characterized the fluorescent properties of these two proteins in a broad spectral range (form Ultra-Violet to Visible region). Excitation spectra, lifetimes and polarization spectra show significant energy transfer from aromatic amino acids to the fluorescent protein chromophore. High steady-state anisotropy values and the lack of a fast component in anisotropy decays show that the fluorescent protein chromophore is rigidly fixed within the protein structure. Furthermore, we show that the chromophores are not accessible to external quenchers such as acrylamide or potassium iodide (KI), allowing the removal of ‘unwanted’ background in the environment with external quencher, while leaving the Cerulean/Venus fluorescence unchanged.
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