Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings.
Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings.
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DOI：
10.1016/j.xpro.2021.100922
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Xiong Y
中科院分区：
文献类型：
--
作者：
Liu Y;Xiong Y
雷帕霉素靶蛋白（TOR）是营养信号传导的中心调节因子。然而，自养植物产生碳相关营养素和氮相关营养素的先天能力使得研究TOR途径变得困难。在这里，我们描述了一个协议的液体培养系统的内源性碳/氮营养物质在拟南芥幼苗的有效消耗。可以提供外源性碳/氮来剖析TOR途径。有关本方案使用和执行的完整详细信息，请参见和。拟南芥内源碳/氮消耗的液体培养系统外源碳/氮可用于剖析营养-TOR信号营养调控根/茎尖细胞增殖依赖于TOR激酶雷帕霉素（rapamycin，TOR）的靶标是营养信号的中心调节子。然而，自养植物产生碳相关营养素和氮相关营养素的先天能力使得研究TOR途径变得困难。在这里，我们描述了一个协议的液体培养系统的内源性碳/氮营养物质在拟南芥幼苗的有效消耗。可以提供外源性碳/氮来剖析TOR途径。
Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution of this protocol, please refer to and. Liquid culture system for depletion of endogenous carbon/nitrogen in Arabidopsis Exogenous carbon/nitrogen can be supplied to dissect the nutrient-TOR signaling Nutrients regulated cell proliferation in root/shoot apex depends on TOR kinase Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway.
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影响因子：
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DOI：
10.1073/pnas.1618782114
发表时间：
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影响因子：
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作者：
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通讯作者：
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