The neutral and acidic glyceroglucolipids from gastric mucous barrier of rat-stomach antrum, body and forestomach.
The neutral and acidic glyceroglucolipids from gastric mucous barrier of rat-stomach antrum, body and forestomach.
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来自大鼠胃窦、胃体和前胃的胃粘膜屏障的中性和酸性甘油糖脂。
DOI：
10.1111/j.1432-1033.1980.tb06101.x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Slomiany,A
中科院分区：
文献类型：
--
作者：
Slomiany,BL;Galicki,NI;Kojima,K;Slomiany,A
研究了甘油糖脂在大鼠胃窦、胃体和前胃粘液屏障中的分布。通过用盐水和2M NaCl对结扎的胃室进行体内灌注，获得表面粘液衬里和预形成的细胞内粘液。从透析和冻干的样品中提取脂质，并在 DEAE-Sephadex 上分离成中性和酸性部分。对每个级分中所含的甘油糖脂进行定量，然后通过薄层色谱法将其分离成各个组分。发现胃的不同区域中甘油糖脂的浓度存在显着差异。胃窦部分的甘油糖脂含量是前胃的五倍。胃窦中硫酸化甘油糖脂的水平是身体的八倍，是前胃的四倍。胃窦灌流液中存在一种硫酸化甘油糖脂，而身体和前胃含有两种额外的酸性甘油糖脂。基于部分酸和碱甲醇分解、高碘酸盐和三氧化铬氧化以及天然和脱硫化合物的甲基化数据，窦甘油糖脂的结构被提议为：SO3H(→6)Glc(α1→6)Glc(α1→6)Glc(α1→6)Glc(α1→3)-1(3)-O-烷基-2-O-酰基甘油。来自胃每个区域的中性甘油糖脂部分的组成相似，仅观察到其浓度的变化。获得的数据表明，硫酸化甘油糖脂参与保护胃窦的胃粘膜，因为它们的浓度在胃的这个最酸性的部分中最高，该部分也最常暴露于十二指肠反流。
Distribution of glyceroglucolipids in the mucous barrier of rat‐stomach antrum, body and forestomach was investigated. Surface mucus lining and preformed intracellular mucus were obtained by perfusionin vivoof the ligated stomach compartments with saline and 2 M NaCl. Lipids were extracted from dialyzed and lyophilized samples, and separated on DEAE‐Sephadex into neutral and acidic fractions. The glyceroglucolipids contained in each fraction were quantified and then separated into individual components by thin‐layer chromatography. Significant differences in the concentration of glyceroglucolipids in the separated areas of the stomach were found. The content of glyceroglucolipids in the antral portion of the stomach was five‐times greater than that of the forestomach. The level of sulfated glyceroglucolipids was eight‐times greater in the antrum as compared to the body and four‐times greater as compared to the forestomach. One sulfated glyceroglucolipid was present in antral perfusates, whereas the body and forestomach contained two additional acidic glyceroglucolipids. Based on data of partial acid and alkaline methanolyses, oxidation with periodate and chromium trioxide, and methylation of the native and desulfated compound, the structure of antral glyceroglucolipid is proposed to be: SO3H(→6)Glc(α1→6)Glc(α1→6)Glc(α1→6)Glc(α1→3)‐1(3)‐O‐alkyl‐2‐O‐acylglycerol. The neutral glyceroglucolipid fractions from each area of the stomach were similar in composition and only variations in their concentration were observed.The data obtained suggest that the sulfated glyceroglucolipids are involved in the protection of the gastric mucosa of the antrum, since their concentration is greatest in this most acidic portion of the stomach which is also most often exposed to duodenal reflux.
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胃肠道损伤和疾病中粘液生物合成、产生和分泌的紊乱。
影响因子：
--
作者：
G. Glass;B. Slomiany
通讯作者：
B. Slomiany
人类唾液的甘油糖脂。
DOI：
--
发表时间：
1978
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
B. Slomiany;A. Slomiany;G. Glass
通讯作者：
G. Glass
刀豆球蛋白 A 与人类分泌物中的血型物质糖蛋白的相互作用。
影响因子：
4.1
作者：
A. Clarke;M. Denborough
通讯作者：
M. Denborough
DOI：
--
发表时间：
1979
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
B. Slomiany;F. Smith;A. Slomiany
通讯作者：
A. Slomiany
血浆中血红蛋白测定联苯胺法的进一步改进；
DOI：
--
发表时间：
1960
期刊：
Journal of Laboratory and Clinical Medicine
影响因子：
--
作者：
G. E. Hanks;M. Cassell;R. Ray;H. Chaplin
通讯作者：
H. Chaplin