Sonochemical effects on fabrication, characterization and antioxidant activities of β-lactoglobulin-chlorogenic acid conjugates.
Sonochemical effects on fabrication, characterization and antioxidant activities of β-lactoglobulin-chlorogenic acid conjugates.
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声化学对β-乳球蛋白-绿原酸缀合物的制造、表征和抗氧化活性的影响
DOI：
10.1016/j.ultsonch.2022.106240
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发表时间：
2023-01
影响因子：
8.4
通讯作者：
Gong, Jinyan
中科院分区：
文献类型：
--
作者：
Liu, Jiayuan;Song, Gongshuai;Zhou, Like;Yuan, Yawen;Wang, Danli;Yuan, Tinglan;Li, Ling;He, Guanghua;Xiao, Gongnian;Chen, Feng;Gong, Jinyan
研究了超声作用下β - 乳球蛋白与绿原酸的相互作用。
当β - 乳球蛋白和绿原酸同时进行超声处理时，会生成共价复合物。
采用超声/氧化还原对法时，观察到复合物的结合量最高。
超声处理提高了复合物的抗氧化性能，但降低了其热稳定性。
探索了通过超声法、氧化还原对法及其组合来形成β - 乳球蛋白 - 绿原酸（LG - CA）结合物。超声处理使用的是带有6毫米探头的探头式超声仪，功率为270瓦，频率为20 - 25千赫兹。通过SDS - PAGE（十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳）确认了结合物的形成，表现为分子量增大。此外，傅里叶红外光谱（FTIR）和圆二色光谱（CD）表明LG - CA结合物的二级结构发生了变化。共价复合物形成后，LG的α - 螺旋和β - 折叠含量降低，无序结构含量显著增加。另外，超声处理及其与氧化还原对法的组合均能显著提高LG的抗氧化性能。前者提高到23.16微摩尔特洛克斯/克样品，后者提高到82 - 106微摩尔特洛克斯/克样品。因此，超声处理既可以单独使用，也可以与氧化还原对法结合使用，以制备具有更强抗氧化活性的LG - CA结合物。
The interaction between β-lactoglobulin and Chlorogenic acid by ultrasonication was studied. Covalent complexes were produced when β-lactoglobulin and Chlorogenic acid were sonicated at the same time. The highest binding content of complexes was observed with the ultrasonication/redox-pair method. Ultrasonication improved the antioxidant properties but reduced the thermal stability of the complex. The β-lactoglobulin-chlorogenic acid (LG-CA) conjugate was explored to be formed through ultrasonication, redox-pair method and their combination, the ultrasonication used a probe ultrasonic machine with a 6 mm probe at 270 W, and the frequency was 20–25 kHz. The formation of the conjugate was confirmed by SDS-PAGE with a larger molecular weight. Besides, Fourier infrared spectroscopy (FTIR) and Circular dichroism (CD) indicated changes in the secondary structure of the LG-CA conjugate. The α-helix and β-sheet contents of LG decreased and the unordered content increased significantly after the formation of covalent complexes. In addition, both the ultrasonic treatment and its combination with redox-pair method could significantly improve the antioxidant properties of LG. The former increased to 23.16 μmol Trolox/g sample, the latter 82–106 μmol Trolox/g sample. Therefore, ultrasonication could be used both individually and in combination with the redox-pair method to produce LG-CA conjugates with stronger antioxidant activities.
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用于非共价纳米复合物的β-乳球蛋白和绿原酸的超声辅助组装：制造、表征和潜在的生物学功能
影响因子：
8.4
作者：
Liu, Jiayuan;Song, Gongshuai;Yuan, Yawen;Zhou, Like;Wang, Danli;Yuan, Tinglan;Li, Ling;He, Guanghua;Yang, Qingyu;Xiao, Gongnian;Gong, Jinyan
通讯作者：
Gong, Jinyan
影响因子：
10.7
作者：
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通讯作者：
Ma, Haile
影响因子：
6.1
作者：
Li, Yihe;Zhang, Zhaoli;Ma, Haile
通讯作者：
Ma, Haile
DOI：
10.1016/bs.afnr.2021.02.011
发表时间：
2021-01-01
期刊：
APPLICATION OF POLYPHENOLS IN FOODS AND FOOD MODELS
影响因子：
--
作者：
Benjakul, Soottawat;Singh, Avtar;Mittal, Ajay
通讯作者：
Mittal, Ajay
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影响因子：
4.3
作者：
Mei, Yimeng;Sun, Huili;Lyu, Deguo
通讯作者：
Lyu, Deguo