A small RNA encoded in the Rv2660c locus of Mycobacterium tuberculosis is induced during starvation and infection.
A small RNA encoded in the Rv2660c locus of Mycobacterium tuberculosis is induced during starvation and infection.
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DOI：
10.1371/journal.pone.0080047
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Arnvig KB
中科院分区：
文献类型：
--
作者：
Houghton J;Cortes T;Schubert O;Rose G;Rodgers A;De Ste Croix M;Aebersold R;Young DB;Arnvig KB
Rv2660c 基因座响应结核分枝杆菌 H37Rv 饥饿而增强的转录促进了将预测的 Rv2660c 蛋白添加到改进的疫苗制剂中。使用链特异性RNA测序，我们表明上调的转录物实际上是在与注释的Rv2660c相反的链上编码的小RNA。该转录本起源于原噬菌体，并且仅在携带 PhiRv2 的菌株中表达。小RNA包含宿主和噬菌体序列，并提供有用的生物标志物来监测感染和/或非复制持续期间的细菌饥饿。使用不同的方法，我们没有在 mRNA 或蛋白质水平上发现 Rv2660c 的任何证据。进一步努力了解 Rv2660c 提高 H56 疫苗功效的机制可能会为结核病的病理学和免疫学提供见解。
Enhanced transcription of the Rv2660c locus in response to starvation of Mycobacterium tuberculosis H37Rv encouraged addition of the predicted Rv2660c protein to an improved vaccine formulation. Using strand-specific RNA sequencing, we show that the up-regulated transcript is in fact a small RNA encoded on the opposite strand to the annotated Rv2660c. The transcript originates within a prophage and is expressed only in strains that carry PhiRv2. The small RNA contains both host and phage sequences and provides a useful biomarker to monitor bacterial starvation during infection and/or non-replicating persistence. Using different approaches we do not find any evidence of Rv2660c at the level of mRNA or protein. Further efforts to understand the mechanism by which Rv2660c improves efficacy of the H56 vaccine are likely to provide insights into the pathology and immunology of tuberculosis.
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影响因子：
3.3
作者：
Rose G;Cortes T;Comas I;Coscolla M;Gagneux S;Young DB
通讯作者：
Young DB
影响因子：
3.7
作者：
Pellin D;Miotto P;Ambrosi A;Cirillo DM;Di Serio C
通讯作者：
Di Serio C
DOI：
10.1074/mcp.m112.018846
发表时间：
2013-05
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Albrethsen J;Agner J;Piersma SR;Højrup P;Pham TV;Weldingh K;Jimenez CR;Andersen P;Rosenkrands I
通讯作者：
Rosenkrands I
影响因子：
30.8
作者：
Supply, Philip;Marceau, Michael;Mangenot, Sophie;Roche, David;Rouanet, Carine;Khanna, Varun;Majlessi, Laleh;Criscuolo, Alexis;Tap, Julien;Pawlik, Alexandre;Fiette, Laurence;Orgeur, Mickael;Fabre, Michel;Parmentier, Cecile;Frigui, Wafa;Simeone, Roxane;Boritsch, Eva C.;Debrie, Anne-Sophie;Willery, Eve;Walker, Danielle;Quail, Michael A.;Ma, Laurence;Bouchier, Christiane;Salvignol, Gregory;Sayes, Fadel;Cascioferro, Alessandro;Seemann, Torsten;Barbe, Valerie;Locht, Camille;Gutierrez, Maria-Cristina;Leclerc, Claude;Bentley, Stephen D.;Stinear, Timothy P.;Brisse, Sylvain;Medigue, Claudine;Parkhill, Julian;Cruveiller, Stephane;Brosch, Roland
通讯作者：
Brosch, Roland
影响因子：
2.8
作者：
Gengenbacher, Martin;Rao, Srinivasa P. S.;Dick, Thomas
通讯作者：
Dick, Thomas