Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.
Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.
复制标题
通过与CDC45和杜松子蛋白缔合对MCM2-7解旋酶的激活。
DOI：
10.1016/j.molcel.2009.12.030
复制
发表时间：
2010-01-29
期刊：
影响因子：
16
通讯作者：
Botchan MR
中科院分区：
文献类型：
--
作者：
Ilves I;Petojevic T;Pesavento JJ;Botchan MR
MCM2-7蛋白在真核生物染色体DNA复制叉上提供必要的解旋酶功能。在细胞周期的G1期，它们仍然装载在DNA上，但不活跃。我们使用重组方法证明果蝇MCM2-7解旋酶与Cdc45和四种GINS蛋白（CMG复合物）复合物激活。MCM AAA+马达的生化活性通过这种关联被改变和增强：ATP水解率提高了两个数量级，解旋酶在圆形模板上的活性很强，对DNA底物的亲和力得到了提高。GINS蛋白有助于DNA底物亲和力，并特异性结合MCM4亚基。GINS、mcm和Cdc45之间的所有成对关联都被检测到，但紧密关联仅在CMG中发生。因此，DNA复制和解绕的开始可能是通过受这些辅助因素关联影响的MCM2-7的变构变化发生的。
MCM2-7 proteins provide essential helicase functions in eukaryotes at chromosomal DNA replication forks. During the G1 phase of the cell cycle they remain loaded on DNA but are inactive. We have used recombinant methods to show that the Drosophila MCM2-7 helicase is activated in complex with Cdc45 and the four GINS proteins (CMG complex). Biochemical activities of the MCM AAA+ motor are altered and enhanced through such associations: ATP hydrolysis rates are elevated by two orders of magnitude, helicase activity is robust on circular templates, and affinity for DNA substrates is improved. The GINS proteins contribute to DNA substrate affinity and bind specifically to the MCM4 subunit. All pair-wise associations between GINS, MCMs and Cdc45 were detected but tight association takes place only in the CMG. The onset of DNA replication and unwinding may thus occur through allosteric changes in MCM2-7 affected by the association of these ancillary factors.
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影响因子：
10.5
作者：
Kubota, Y;Takase, Y;Takisawa, H
通讯作者：
Takisawa, H
影响因子：
21.3
作者：
Gambus, A;Jones, RC;Labib, K
通讯作者：
Labib, K
DOI：
10.1073/pnas.0911500106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Evrin, Cecile;Clarke, Pippa;Speck, Christian
通讯作者：
Speck, Christian
CDC45-MCM-GINS，是DNA复制的新功率播放器。
影响因子：
2.3
作者：
Aparicio T;Ibarra A;Méndez J
通讯作者：
Méndez J
影响因子：
16
作者：
Pacek, M;Tutter, AV;Walter, JC
通讯作者：
Walter, JC