Suppressive regulatory T cells and latent transforming growth factor-β-expressing macrophages are altered in the peritoneal fluid of patients with endometriosis.
Suppressive regulatory T cells and latent transforming growth factor-β-expressing macrophages are altered in the peritoneal fluid of patients with endometriosis.
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DOI：
10.1186/s12958-018-0325-2
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发表时间：
2018-02-01
期刊：
影响因子：
--
通讯作者：
Murakami T
中科院分区：
文献类型：
--
作者：
Hanada T;Tsuji S;Nakayama M;Wakinoue S;Kasahara K;Kimura F;Mori T;Ogasawara K;Murakami T
子宫内膜异位症是一种已知的不孕原因。调节性T细胞（Tregs）和转化生长因子-β (TGF-β)引起的免疫耐受差异被认为与子宫内膜异位症的病理有关。有证据表明，Tregs可以分为三个功能和表型不同的亚群，激活的TGF-β从特定细胞表面的潜伏期相关肽（LAP）释放。本研究的目的是检查有和没有子宫内膜异位症患者外周血（PB）和腹膜液（PF）中Treg亚群和LAP的差异。收集28例经腹腔镜和组织病理学诊断为子宫内膜异位症的妇女和20例经腹腔镜手术的无子宫内膜异位症妇女的PB和PF。利用特异性单克隆抗体，流式细胞术分析了表达LAP的CD4+ T淋巴细胞（CD45RA+FoxP3low静息Tregs、CD45RA−FoxP3low高效Tregs和CD45RA−FoxP3low非Tregs）和CD11b+单核细胞亚群。子宫内膜异位症患者PF样本中抑制性Tregs（静息Tregs和效应Tregs）的比例显著高于对照组（P分别为0.02和P < 0.01），而PB样本与对照组之间无差异。子宫内膜异位症患者PF组CD11b+LAP+巨噬细胞比例显著低于对照组（P < 0.01）， PB组无明显变化。在子宫内膜异位症患者的PF中，抑制性Tregs和LAP+巨噬细胞的比例发生局部改变。本文的在线版本（10.1186/s12958-018-0325-2）包含补充资料，仅供授权用户使用。
Endometriosis is a known cause of infertility. Differences in immune tolerance caused by regulatory T cells (Tregs) and transforming growth factor-β (TGF-β) are thought to be involved in the pathology of endometriosis. Evidence has indicated that Tregs can be separated into three functionally and phenotypically distinct subpopulations and that activated TGF-β is released from latency-associated peptide (LAP) on the surfaces of specific cells. The aim of this study was to examine differences in Treg subpopulations and LAP in the peripheral blood (PB) and peritoneal fluid (PF) of patients with and without endometriosis. PB and PF were collected from 28 women with laparoscopically and histopathologically diagnosed endometriosis and 20 disease-free women who were subjected to laparoscopic surgery. Three subpopulations of CD4+ T lymphocytes (CD45RA+FoxP3low resting Tregs, CD45RA−FoxP3high effector Tregs, and CD45RA−FoxP3low non-Tregs) and CD11b+ mononuclear cells expressing LAP were analyzed by flow cytometry using specific monoclonal antibodies. Proportions of suppressive Tregs (resting and effector Tregs) were significantly higher in the PF samples of patients with endometriosis than in those of control women (P = 0.02 and P < 0.01, respectively) but did not differ between the PB samples of patients and controls. The percentage of CD11b+LAP+ macrophages was significantly lower in PF samples of patients with endometriosis than in those of controls (P < 0.01) but was not altered in the PB samples. Proportions of suppressive Tregs and LAP+ macrophages are altered locally in the PF of endometriosis patients. The online version of this article (10.1186/s12958-018-0325-2) contains supplementary material, which is available to authorized users.
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影响因子：
15.3
作者：
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影响因子：
2.1
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影响因子：
3.4
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15.9
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