A Real-Time PCR Assay for the Detection of a penA Mutation Associated with Ceftriaxone Resistance in Neisseria gonorrhoeae.
A Real-Time PCR Assay for the Detection of a penA Mutation Associated with Ceftriaxone Resistance in Neisseria gonorrhoeae.
复制标题
实时 PCR 检测与淋病奈瑟菌头孢曲松耐药性相关的 penA 突变检测。
DOI：
10.1016/j.jgar.2019.02.011
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ohnishi M.
中科院分区：
文献类型：
--
作者：
Shimuta K;Igawa G;Yasuda M;Deguchi T;Nakayama SI;Ohnishi M.
目的头孢曲松（CRO）耐药性在全球范围内蔓延，阻碍了淋球菌感染的有效治疗。为了提高淋病奈瑟菌（N. gonorrhoeae）耐cro菌株的耐药性监测，本研究建立了淋病奈瑟菌基因组DNA检测系统。方法采用实时荧光定量PCR法检测新分离的耐cro菌n的抗原。设计了淋病菌株。引物序列和探针序列信息通过neisseriaspp . enpenaof的序列比较得到。andpenAof CRO-resistantN。gonorrhoeaestrains。结果GU140106、FC428和A8806三株菌株的基因组DNA检测结果均为阳性。使用204N基因组DNA对该检测进行评估。gonorrhoeaeand 95 neisseriaspp。已知CRO最低抑制浓度的分离株。对这些菌株进行PCR检测，鉴定出3株与fc428相关的菌株，其中含有60.001的sedpena，其余为201N。淋病菌株和95奈瑟氏球菌。菌株呈阴性。结论建立了一种实时荧光定量pcr检测方法，可检测携带mosaicpenA-59.001 （GU140106）、penA-60.001 （FC428）和pena -64.001 （A8806）等位基因的菌株的基因组DNA，并与n进行区分。gonorrhoeaeandNeisseriaspp。含有其他基因的菌株。
ObjectivesCeftriaxone (CRO) resistance is spreading worldwide, and hindering the effective treatment of gonococcal infections. This study developed a detection system for the genomic DNA of CRO-resistant Neisseria gonorrhoeae (N. gonorrhoeae) strains, in order to improve the surveillance of antimicrobial resistance.MethodsA real-time PCR assay targeting thepenAgene of recently isolated CRO-resistantN. gonorrhoeaestrains was designed. Primer and probe sequence information was obtained from sequence comparisons betweenpenAofNeisseriaspp. andpenAof CRO-resistantN. gonorrhoeaestrains.ResultsUsing this assay, a positive reaction was observed using the genomic DNA of three strains (GU140106, FC428, and A8806). The assay was evaluated using genomic DNA of 204N. gonorrhoeaeand 95Neisseriaspp. isolates with known minimum inhibitory concentrations of CRO. Following PCR assays for these strains, three FC428-related strains were positively identified, which possessedpenA-60.001, whereas the remaining 201N. gonorrhoeaestrains and 95Neisseriaspp. strains were negative.ConclusionsA real-time PCR-based assay was designed to detect the genomic DNA of strains harbouring mosaicpenA-59.001 (GU140106),penA-60.001 (FC428), andpenA-64.001 (A8806) alleles and to discriminate them fromN. gonorrhoeaeandNeisseriaspp. strains harbouring other genes.
登录
查看更多内容
影响因子：
3.7
作者：
Unemo M
通讯作者：
Unemo M
PCR直接检测头孢曲松耐药淋病奈瑟菌FC428株相关penA基因
影响因子：
11.8
作者：
D. Whiley;L. Mhango;A. Jennison;G. Nimmo;Monica Lahra
通讯作者：
Monica Lahra
影响因子：
5.2
作者：
Unemo, Magnus;Golparian, Daniel;Harris, Simon R.
通讯作者：
Harris, Simon R.
影响因子：
4.9
作者：
Tomberg, Joshua;Unemo, Magnus;Nicholas, Robert A.
通讯作者：
Nicholas, Robert A.
影响因子：
4.9
作者：
Ohnishi, Makoto;Golparian, Daniel;Unemo, Magnus
通讯作者：
Unemo, Magnus