Identification and characterization of three chemosensory receptor families in the cotton bollworm Helicoverpa armigera.
Identification and characterization of three chemosensory receptor families in the cotton bollworm Helicoverpa armigera.
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DOI：
10.1186/1471-2164-15-597
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发表时间：
2014-07-15
期刊：
影响因子：
4.4
通讯作者：
Anderson A
中科院分区：
文献类型：
--
作者：
Liu NY;Xu W;Papanicolaou A;Dong SL;Anderson A
化学感受器包括嗅觉受体(ORs)、味觉受体(GRs)和离子受体(IRs)，它们在感知化学信号和指导昆虫行为方面发挥着核心作用，是害虫防治的潜在靶标基因。棉铃虫是最具破坏性的害虫之一，可以捕食200多种不同的植物。寄主植物的多样性很可能与复杂的化学感觉系统有关。在此，我们在以前工作的基础上，表征了与环境相互作用有关的关键化学感觉组织，包括幼虫触角、幼虫口器和幼虫脂肪体，以及男性和女性成体头部、男性和女性成体眼睑和女性腹部。利用转录组测序、三位一体RNA-seq组装和广泛的人工筛选，我们总共鉴定了91个候选化学感觉受体(60个候选ORs，10个GRs和21个IRS)。这些候选人中有35人提交了完整的成绩单。首先，我们对不同测序的组织进行了电子差异表达分析。此外，我们使用逆转录(RT)-PCR在成虫和幼虫的不同阶段建立了广泛的表达谱。我们发现，HarmOR51的表达谱仅限于成年雄性触角，这表明它在交配中的作用得到了将其聚集到信息素受体分支的系统发育分析的进一步支持。在钙成像分析中，HarmOR51没有显示出对两种棉铃虫性信息素成分(Z9-16：ALD或Z11-16：ALD)的反应，这引发了未来的详细研究。此外，我们还发现了四个新的HarmORs(OR1，53，54和58)，它们似乎是幼虫触角特异的。最后，我们的表达谱显示四个“分歧型”HarmIR(IR2、7d.1、7d.2和7d.3)在成虫和幼虫触角中都有表达，这表明它们与它们在果蝇中的同源物功能不同。这项研究使用精心策划的转录转录方法，结合广泛的表达谱和更有限的功能特征，探索了三个化学受体超家族基因。我们的结果现在已经为研究这种害虫的化学受体互补提供了广泛的资源，同时允许有针对性的实验来确定潜在的害虫控制的分子靶标和研究昆虫与植物的相互作用。本文的在线版本(DOI：10.1186/1471-2164-15-597)包含补充材料，可供授权用户使用。
Chemosensory receptors including olfactory receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) play a central role in sensing chemical signals and guiding insect behaviours, and are potential target genes in insect pest control. The cotton bollworm Helicoverpa armigera is one of the most destructive pest species that can feed on over 200 different plant species. This diversity of host plants is likely linked to a complex chemosensory system. Here we built on previous work to characterize crucial chemosensory tissues linked to environmental interactions including larval antennae, larval mouthparts and larval fat bodies, as well as male and female adult heads, male and female adult tarsi, and female abdomens. Using transcriptome sequencing, Trinity RNA-seq assemblies and extensive manual curation, we identified a total of 91 candidate chemosensory receptors (60 candidate ORs, 10 GRs and 21 IRs). Thirty-five of these candidates present full-length transcripts. First, we performed in silico differential expression analysis on different sequenced tissues. Further, we created extensive expression profiles using reverse transcription (RT)-PCR on a variety of adult and larval stages. We found that the expression profile of HarmOR51 was limited to adult male antenna suggesting a role in mating that was further supported by a phylogenetic analysis clustering it into the pheromone receptor clade. HarmOR51 in calcium imaging analysis did not show responses to either of the two H. armigera sex pheromone components (Z9-16:Ald or Z11-16:Ald) inviting a future detailed study. In addition, we found four novel HarmORs (OR1, 53, 54 and 58) that appeared to be larvae-antennal specific. Finally, our expression profiling showed that four “divergent” HarmIRs (IR2, 7d.1, 7d.2 and 7d.3) were expressed in both adult and larval antennae, suggesting a functional divergence from their Drosophila homologues. This study explored three chemoreceptor superfamily genes using a curated transcriptomic approach coupled with extensive expression profiling and a more limited functional characterization. Our results have now provided an extensive resource for investigating the chemoreceptor complement of this insect pest, and meanwhile allow for targeted experiments to identify potential molecular targets for pest control and to investigate insect-plant interactions. The online version of this article (doi:10.1186/1471-2164-15-597) contains supplementary material, which is available to authorized users.
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