Glia‐to‐axon communication: Enrichment of glial proteins transferred to the squid giant axon
Glia‐to‐axon communication: Enrichment of glial proteins transferred to the squid giant axon
复制标题
神经胶质细胞到轴突的通讯：神经胶质蛋白的富集转移到鱿鱼巨轴突
DOI：
10.1002/jnr.490410305
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发表时间：
1995
影响因子：
4.2
通讯作者：
G. Bittner
中科院分区：
文献类型：
--
作者：
R. A. Sheller;R. A. Sheller;R. A. Sheller;M. Tytell;M. Tytell;M. E. Smyers;G. Bittner
新合成的蛋白质从神经胶质鞘转移到轴突中是鱿鱼巨大轴突的一个有据可查的过程。在这项研究中，我们使用了一种新的方法来分离转移的胶质蛋白（TGPs）的鱿鱼巨轴突的内源性轴浆蛋白。将含有放射性标记的TGPs的轴浆挤出为圆柱体并浸入细胞内缓冲液中。1-30分钟后，TGP在细胞内缓冲液中富集，因为它们从轴浆洗脱到细胞内缓冲液中的速度比内源性轴浆蛋白快得多。当以24，000 g离心20 min时，大多数细胞内缓冲液中富集的TGP不会沉淀，并且在不添加Triton X-100的情况下对蛋白酶消化敏感。此外，透射电子显微镜放射自显影完整的轴突，含有放射性标记的TGPs，表明大多数TGPs与轴突内的囊泡细胞器无关。我们的结论是，大多数TGP不包含在囊泡中的轴浆的鱿鱼巨大的轴突，如预期的，如果神经胶质细胞到轴突转移的机制是传统的胞吐或微吞噬。© 1995 Wiley利斯公司
The transfer of newly synthesized proteins from the glial sheath into the axon is a well‐documented process for the squid giant axon. In this study, we used a novel approach to separate the transferred glial proteins (TGPs) from the endogenous axoplasmic proteins of the squid giant axon. Axoplasm, containing radiolabelled TGPs, was extruded as a cylinder and immersed in an intracellular buffer. After 1–30 min, the TGPs were enriched in the intracellular buffer, because they were eluted from the axoplasm into the intracellular buffer much faster than the endogenous axoplasmic proteins. Most of the TGPs enriched in the intracellular buffer did not pellet when centrifuged at 24,000 g for 20 min and were susceptible to protease digestion without the addition of Triton X‐100. Additionally, transmission electron microscopic autoradiography of intact axons, containing radiolabelled TGPs, suggested that most TGPs were not associated with vesicular organelles within the axon. We conclude that most of the TGPs are not contained within vesicles in the axoplasm of the squid giant axon, as would be expected if the mechanism of glia‐to‐axon transfer were conventional exocytosisendocytosis or microphagocytosis. © 1995 Wiley‐Liss, Inc.
DOI：
10.1016/s0021-9258(18)88868-8
发表时间：
1985-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Lewis;S. Turco;M. Green
通讯作者：
M. Lewis;S. Turco;M. Green
天冬氨酸铅块染色在磷脂定量 EM 放射自显影中的应用：在有髓鞘周围神经中的应用。
DOI：
10.1177/37.9.2475541
发表时间：
1989
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Gould,RM;Armstrong,R
通讯作者：
Armstrong,R
影响因子：
56.9
作者：
BRADY, ST;LASEK, RJ;ALLEN, RD
通讯作者：
ALLEN, RD