Acetylcholinesterase-catalyzed hydrolysis allows ultrasensitive detection of pathogens with the naked eye.

Acetylcholinesterase-catalyzed hydrolysis allows ultrasensitive detection of pathogens with the naked eye.
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乙酰胆碱酯酶催化的水解允许用肉眼超灵敏地检测病原体。

DOI:
10.1002/anie.201307952
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发表时间:
2013-12-23
影响因子:
16.6
通讯作者:
Chen, Xiaoyuan
Chen, Xiaoyuan
中科院分区:
化学1区
文献类型:
--
作者:
Liu, Dingbin;Wang, Zhantong;Jin, Albert;Huang, Xinglu;Sun, Xiaolian;Wang, Fu;Yan, Qiang;Ge, Shengxiang;Xia, Ningshao;Niu, Gang;Liu, Gang;Walker, A. R. Hight;Chen, Xiaoyuan

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本文描述了一种基于乙酰胆碱酯酶催化的水解反应的病原体快速诊断平台。由于采用了信号放大策略,该方法的灵敏度与聚合酶链式反应相当。此外,该分析的读数是基于溶液的颜色变化,仅用肉眼就可以很容易地观察到这一点。
This paper describes a rapid diagnostic platform for pathogen detection based on the acetylcholinesterase-catalyzed hydrolysis reaction. Owing to the signal amplification strategies, the sensitivity of this assay is comparable to that of PCR. In addition, the readout of this assay is based on the color change of solutions, which can be easily observed by the naked eye alone.
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