A novel double-tryptophan peptide pheromone controls competence in Streptococcus spp. via an Rgg regulator.
A novel double-tryptophan peptide pheromone controls competence in Streptococcus spp. via an Rgg regulator.
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DOI：
10.1111/j.1365-2958.2010.07361.x
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发表时间：
2010-11
影响因子：
3.6
通讯作者：
Federle MJ
中科院分区：
文献类型：
--
作者：
Mashburn-Warren L;Morrison DA;Federle MJ
所有的链球菌基因组编码的替代西格玛因子SigX和21 SigX依赖性蛋白所需的遗传转化，但没有化脓性链球菌已知的发展能力。解决这个悖论可能取决于对sigX调控的理解。我们报告了一种与变形链球菌和化脓性链球菌的sigX基因相关的调节回路的鉴定，该回路使用一种新型的小的、含双色氨酸的能力诱导肽（CIP）信息素。在这两组中，CIP基因（我们命名为comS）和sigX具有相同的非典型启动子，由9 bp反向重复序列组成，与-10六聚体相隔19 bp。comS与编码Rgg家族的推定转录因子的基因相邻，并受其产物的调节，我们将其命名为ComR。在S中缺失comR或comS。变异株废除了可转化性，缺失寡肽通透酶亚基oppD也是如此，这表明CIP是输入的。提供S.用CIP的合成片段对变异株的研究表明，包括WW基序在内的7个C-末端残基引起sigX和感受态的强烈诱导。我们认为该回路是S.变形链球菌，我们推断，它控制能力在所有化脓性链球菌中以平行的方式。
All streptococcal genomes encode the alternative sigma factor SigX and 21 SigX-dependent proteins required for genetic transformation, yet no pyogenic streptococci are known to develop competence. Resolving this paradox may depend on understanding the regulation of sigX. We report the identification of a regulatory circuit linked to the sigX genes of both mutans and pyogenic streptococci that uses a novel small, double-tryptophan-containing competence-inducing peptide (CIP) pheromone. In both groups, the CIP gene, which we designate comS, and sigX have identical, noncanonical promoters consisting of 9-bp inverted repeats separated from a −10 hexamer by 19 bp. comS is adjacent to a gene encoding a putative transcription factor of the Rgg family and is regulated by its product, which we designate ComR. Deletion of comR or comS in S. mutans abolished transformability, as did deletion of the oligopeptide permease subunit oppD, suggesting that CIP is imported. Providing S. mutans with synthetic fragments of CIP revealed that seven C-terminal residues, including the WW motif, cause robust induction of both sigX and the competent state. We propose that this circuit is the proximal regulator of sigX in S. mutans, and we infer that it controls competence in a parallel way in all pyogenic streptococci.
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影响因子：
3.6
作者：
Ansaldi, M;Marolt, D;Dubnau, D
通讯作者：
Dubnau, D
影响因子：
2.6
作者：
Gromkova, RC;Mottalini, TC;Dove, MG
通讯作者：
Dove, MG
DOI：
10.1073/pnas.91.8.3280
发表时间：
1994-04-12
影响因子：
11.1
作者：
BESSEN, DE;HOLLINGSHEAD, SK
通讯作者：
HOLLINGSHEAD, SK
影响因子：
3.2
作者：
Fontaine, Laetitia;Boutry, Celiine;Hols, Pascal
通讯作者：
Hols, Pascal
影响因子：
5.6
作者：
Bae, T;Kozlowicz, B;Dunny, GM
通讯作者：
Dunny, GM