Shenfu Injection: A Famous Chinese Prescription That Promotes HCN4 Activity in Bone Marrow Mesenchymal Stem Cells.
Shenfu Injection: A Famous Chinese Prescription That Promotes HCN4 Activity in Bone Marrow Mesenchymal Stem Cells.
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DOI：
10.1155/2021/9912844
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Li R
中科院分区：
文献类型：
--
作者：
Zhao X;Chu Q;Wu W;Wu H;Wang S;Qing L;Zhou X;Luo Z;Kang L;Li R
观察参附注射液对骨髓间充质干细胞（BMSCs）HCN 4活性的影响。将BMSCs样品分为6组：对照组、SFI高剂量组（0.25 ml/ml）、SFI中剂量组（0.1 ml/ml）、SFI低剂量组（0.05 ml/ml）、腺病毒编码对照载体组和腺病毒编码HCN 4组。透射电镜观察细胞超微结构。采用定量逆转录PCR（RT-qPCR）检测HCN 4的表达，采用全细胞膜片钳技术检测HCN 4的活性。采用酶联免疫吸附试验检测cAMP含量。流式细胞仪检测证实分离的细胞具有BMSC样表型。根据细胞超微结构，SFI和腺病毒编码HCN 4组的BMSCs发生分化。全细胞膜片钳技术显示，参附注射液可激活BMSCs的内向起搏电流，并呈浓度依赖性。RT-qPCR结果显示，参附注射液高剂量组HCN 4表达明显高于中、低剂量组，而过表达HCN 4组cAMP含量明显降低;参附注射液高剂量组cAMP含量明显升高。总之，SFI促进BMSCs中的HCN 4活性，这可以解释其对心血管疾病患者的治疗效果。
We investigated the effects of Shenfu Injection (SFI) on HCN4 activity in bone marrow mesenchymal stem cells (BMSCs). The sample of BMSCs was divided into six groups: a control group, a high-dose SFI group (0.25 ml/ml), a middle-dose SFI group (0.1 ml/ml), a low-dose SFI group (0.05 ml/ml), an adenovirus-encoded control vector group, and an adenovirus-encoded HCN4 group. Cell ultrastructure was observed using a transmission electron microscope. Quantitative reverse transcription PCR (RT-qPCR) was performed to detect HCN4 expression, and HCN4 activity was detected using the whole-cell patch clamp technique. An enzyme-linked immunosorbent assay was performed to detect cAMP content. Application of flow cytometry confirmed that the isolated cells showed BMSC-like phenotypes. Differentiation of BMSCs in both the SFI and the adenovirus-encoding HCN4 groups occurred according to the cellular ultrastructure. Application of the whole-cell patch clamp technique revealed that SFI could activate the inward pacing current of BMSCs in a concentration-dependent manner. The RT-qPCR results showed that HCN4 expression was significantly higher in the high-dose SFI group than in the medium- and low-dose groups, whereas the cAMP content in the overexpressed HCN4 group decreased significantly; this content in the high-dose SFI group increased significantly. In conclusion, SFI promotes HCN4 activity in BMSCs, which could explain its treatment effect when administered to patients with cardiovascular diseases.
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DOI：
10.1186/s12964-015-0124-8
发表时间：
2016-01-12
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
L Ramos T;Sánchez-Abarca LI;Muntión S;Preciado S;Puig N;López-Ruano G;Hernández-Hernández Á;Redondo A;Ortega R;Rodríguez C;Sánchez-Guijo F;del Cañizo C
通讯作者：
del Cañizo C
影响因子：
--
作者：
Bagliani, Giuseppe;Leonelli, Fabio;Padeletti, Luigi
通讯作者：
Padeletti, Luigi
影响因子：
5.4
作者：
Li Y;Yang M;Zhang G;Li L;Ye B;Huang C;Tang Y
通讯作者：
Tang Y
影响因子：
13.6
作者：
Vedantham V
通讯作者：
Vedantham V
影响因子：
4.5
作者：
Chauveau, Samuel;Brink, Peter R.;Cohen, Ira S.
通讯作者：
Cohen, Ira S.